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Cell Stress and Chaperones

, Volume 21, Issue 5, pp 783–791 | Cite as

Effect of resistance exercise training on expression of Hsp70 and inflammatory cytokines in skeletal muscle and adipose tissue of STZ-induced diabetic rats

  • M. Molanouri ShamsiEmail author
  • M. Mahdavi
  • L. S. Quinn
  • R. Gharakhanlou
  • A. Isanegad
Original Paper

Abstract

Impairment of adipose tissue and skeletal muscles accrued following type 1 diabetes is associated with protein misfolding and loss of adipose mass and skeletal muscle atrophy. Resistance training can maintain muscle mass by changing both inflammatory cytokines and stress factors in adipose tissue and skeletal muscle. The purpose of this study was to determine the effects of a 5-week ladder climbing resistance training program on the expression of Hsp70 and inflammatory cytokines in adipose tissue and fast-twitch flexor hallucis longus (FHL) and slow-twitch soleus muscles in healthy and streptozotocin-induced diabetic rats. Induction of diabetes reduced body mass, while resistance training preserved FHL muscle weight in diabetic rats without any changes in body mass. Diabetes increased Hsp70 protein content in skeletal muscles, adipose tissue, and serum. Hsp70 protein levels were decreased in normal and diabetic rats by resistance training in the FHL, but not soleus muscle. Furthermore, resistance training decreased inflammatory cytokines in FHL skeletal muscle. On the other hand, Hsp70 and inflammatory cytokine protein levels were increased by training in adipose tissue. Also, significant positive correlations between inflammatory cytokines in adipose tissue and skeletal muscles with Hsp70 protein levels were observed. In conclusion, we found that in diabetic rats, resistance training decreased inflammatory cytokines and Hsp70 protein levels in fast skeletal muscle, increased adipose tissue inflammatory cytokines and Hsp70, and preserved FHL muscle mass. These results suggest that resistance training can maintain skeletal muscle mass in diabetes by changing inflammatory cytokines and stress factors such as Hsp70 in skeletal muscle and adipose tissue.

Keywords

Hsp70 Inflammatory cytokines Skeletal muscle Adipose tissue Resistance training Type 1diabetes 

Notes

Acknowledgments

This work was supported by the Research Center of Tarbiat Modarres University (TMU), Tehran, Iran. We wish to thank Professor Yaghob Fathoallahy for their kind help and sincere cooperation.

Compliance with ethical standards

All experiments involving animals were conducted according to the policies of the Iranian Convention for the Protection of Vertebrate Animals Used for Experimental and other Scientific Purposes, and the protocol was approved by the Ethics Committee of the School of Medicine Sciences, Tarbiat Modares University (TMU), Tehran, Iran.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Cell Stress Society International 2016

Authors and Affiliations

  • M. Molanouri Shamsi
    • 1
    Email author
  • M. Mahdavi
    • 2
  • L. S. Quinn
    • 3
  • R. Gharakhanlou
    • 1
  • A. Isanegad
    • 4
    • 5
  1. 1.Physical Education and Sport Sciences Dept., Faculty of HumanitiesTarbiat Modares UniversityTehranIran
  2. 2.Immunology DepartmentPasteur Institute of IranTehranIran
  3. 3.Research Service, VA Puget Sound Health Care System, and Division of Gerontology and Geriatric Medicine, Department of MedicineUniversity of WashingtonSeattleUSA
  4. 4.Physical Education and Sport Sciences Dept., Faculty of HumanitiesShahed UniversityTehranIran
  5. 5.Immunoregulation Research CenterShahed UniversityTehranIran

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