Investigation of immunomodulatory and anti-inflammatory effects of eriodictyol through its cellular anti-oxidant activity
Many studies have been performed to assess the potential utility of natural products as immunomodulatory agents to enhance host responses against infection or to ameliorate immune-based pathologies. To determine whether eriodictyol has immunomodulatory effects and clarify which types of immune effector cells are stimulated in vitro, we investigated the stimulatory effect of eriodictyol on spleen cells isolated from BALB/c mice. Eriodictyol significantly stimulated splenocyte proliferation. However, only B lymphocytes (not T lymphocytes) could be stimulated by eriodictyol in a dose-related manner. Studies assessing potential effect of eriodictyol on innate immunity reported that eriodictyol enhanced significantly the killing activity of natural killer (NK) cells, T lymphocytes, and macrophages. We also demonstrated that eriodictyol inhibited nitric oxide (NO) production and lysosomal enzyme activity in murine peritoneal macrophages cultured ex-vivo, suggesting a potential anti-inflammatory effect in situ. Eriodictyol revealed also a cellular anti-oxidant activity in splenocytes and macrophages. Furthermore, eriodictyol increased catalase activity in spleen cells. From this data, it can be concluded that eriodictyol exhibited an immunomodulatory effect that could be ascribed in part to a cytoprotective effect related to its anti-oxidant activity.
KeywordsEriodictyol Immunomodulation Cellular anti-oxidant activity
The authors acknowledge the “Ministère Tunisien de l’enseignement supérieur et de la recherché scientifique” for financial support of this study.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- Ismail N, Abdullah H, Seidel V, Rotondo D (2012) Human natural killer (NK) cell activation by luteolin from Brucea javanica leaves. J. Cancer Res. Exp. Oncol. ISSN: 2141–2243Google Scholar
- Sharififar F, Pournourmohammadi SH, Arabnezhad M, Rastegarianzadeh R, Ranjbaran O, Pourhemati A (2009) Immunomodulatory activity of aqueous extract of Heracleum persicum Desf. Mice Iran J Pharm Res 8:287–292Google Scholar