Cell Stress and Chaperones

, Volume 21, Issue 5, pp 773–781 | Cite as

Investigation of immunomodulatory and anti-inflammatory effects of eriodictyol through its cellular anti-oxidant activity

  • Imen Mokdad-Bzeouich
  • Nadia Mustapha
  • Aicha Sassi
  • Ahmed Bedoui
  • Mohamed Ghoul
  • Kamel Ghedira
  • Leila Chekir-Ghedira
Original Paper


Many studies have been performed to assess the potential utility of natural products as immunomodulatory agents to enhance host responses against infection or to ameliorate immune-based pathologies. To determine whether eriodictyol has immunomodulatory effects and clarify which types of immune effector cells are stimulated in vitro, we investigated the stimulatory effect of eriodictyol on spleen cells isolated from BALB/c mice. Eriodictyol significantly stimulated splenocyte proliferation. However, only B lymphocytes (not T lymphocytes) could be stimulated by eriodictyol in a dose-related manner. Studies assessing potential effect of eriodictyol on innate immunity reported that eriodictyol enhanced significantly the killing activity of natural killer (NK) cells, T lymphocytes, and macrophages. We also demonstrated that eriodictyol inhibited nitric oxide (NO) production and lysosomal enzyme activity in murine peritoneal macrophages cultured ex-vivo, suggesting a potential anti-inflammatory effect in situ. Eriodictyol revealed also a cellular anti-oxidant activity in splenocytes and macrophages. Furthermore, eriodictyol increased catalase activity in spleen cells. From this data, it can be concluded that eriodictyol exhibited an immunomodulatory effect that could be ascribed in part to a cytoprotective effect related to its anti-oxidant activity.


Eriodictyol Immunomodulation Cellular anti-oxidant activity 



The authors acknowledge the “Ministère Tunisien de l’enseignement supérieur et de la recherché scientifique” for financial support of this study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Cell Stress Society International 2016

Authors and Affiliations

  • Imen Mokdad-Bzeouich
    • 1
    • 2
  • Nadia Mustapha
    • 1
    • 2
  • Aicha Sassi
    • 1
    • 2
  • Ahmed Bedoui
    • 1
    • 2
  • Mohamed Ghoul
    • 3
  • Kamel Ghedira
    • 2
  • Leila Chekir-Ghedira
    • 1
    • 2
  1. 1.Laboratory of Cellular and Molecular Biology, Faculty of Dental MedicineUniversity of MonastirMonastirTunisia
  2. 2.Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of PharmacyUniversity of MonastirMonastirTunisia
  3. 3.Laboratory of Biomolecular Engineering, National School of Agronomy and Food IndustriesNational Polytechnics Institute of Lorraine ENSAIA-INPLNancyFrance

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