Cell Stress and Chaperones

, Volume 20, Issue 1, pp 159–168 | Cite as

Hsp70 and lipid peroxide levels following heat stress in Xeropicta derbentina (Krynicki 1836) (Gastropoda, Pulmonata) with regard to different colour morphs

  • A. Dieterich
  • S. Troschinski
  • S. Schwarz
  • M. A. Di Lellis
  • A. Henneberg
  • U. Fischbach
  • M. Ludwig
  • U. Gärtner
  • R. Triebskorn
  • H.-R. Köhler
Original Paper


Terrestrial snails which live under dry and hot conditions need efficient mechanisms of adaptation to counteract the problems of desiccation and over-heating. A profoundly heat tolerant snail species is the Mediterranean Xeropicta derbentina, exhibiting different shell colour morphs ranging from pale white to darkly banded. Considering that dark-pigmented snails are believed to have a disadvantage due to faster heating, we investigated possible differences in the stress markers Hsp70 and lipid peroxideation between four pre-defined colour morphs which were exposed to different temperatures for eight hours. The highest Hsp70 levels were observed in response to 38-40 °C. Levels decreased when this temperature was exceeded. Snails of a pre-defined colour category 3 (with a large black band at the umbilicus side of the shell) showed the most prominent Hsp70 response. Lipid peroxideation levels also showed a maximum at 38 °C but displayed a second peak at rather high temperatures at which the Hsp70 level already had decreased (45-48 °C). Particularly pure white snails (category 1) and the most pigmented ones (category 4) were found to have different levels of lipid peroxidation at 38 °C and 45 °C compared to the other morphs. A hypothesis involving a combined two-phase defence mechanism, to which both, the Hsp70 protection system and the antioxidant defence system, may contribute, is discussed.


FOX assay Heat stress Hsp70 level Lipid peroxidation Shell colouration 



The authors would like to thank Christophe Mazzia, University of Avignon, for the provision of our sampling site in Modène. The study was funded by the German Research Council (DFG, Ko 1978/5-3) and the Twinning Projects Grant of Tübingen University. The authors would like to thank two unknown reviewers for their useful comments on an earlier version of this manuscript.


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Copyright information

© Cell Stress Society International 2014

Authors and Affiliations

  • A. Dieterich
    • 1
  • S. Troschinski
    • 1
  • S. Schwarz
    • 1
  • M. A. Di Lellis
    • 1
  • A. Henneberg
    • 1
  • U. Fischbach
    • 2
  • M. Ludwig
    • 2
  • U. Gärtner
    • 2
  • R. Triebskorn
    • 1
  • H.-R. Köhler
    • 1
  1. 1.Animal Physiological Ecology, University of TübingenTübingenGermany
  2. 2.Institute of Applied ResearchUniversity of Applied SciencesEsslingenGermany

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