Cell Stress and Chaperones

, Volume 18, Issue 5, pp 661–665 | Cite as

Butyric acid retention in gingival tissue induces oxidative stress in jugular blood mitochondria

  • Marni E. Cueno
  • Kenichi Imai
  • Noriko Matsukawa
  • Takamitsu Tsukahara
  • Tomoko Kurita-Ochiai
  • Kuniyasu Ochiai
Short Communication


Butyric acid (BA) is a major extracellular metabolite produced by anaerobic periodontopathic bacteria and is commonly deposited in the gingival tissue. BA induces mitochondrial oxidative stress in vitro; however, its effects in vivo were never elucidated. Here, we determined the effects of butyric acid retention in the gingival tissues on oxidative stress induction in the jugular blood mitochondria. We established that BA injected in the rat gingival tissue has prolonged retention in gingival tissues. Blood taken at 0, 60, and 180 min after BA injection was used for further analysis. We isolated blood mitochondria, verified its purity, and measured hydrogen peroxide (H2O2), heme, superoxide (SOD), and catalase (CAT) to determine BA effects. We found that H2O2, heme, SOD, and CAT levels all increased after BA injection. This would insinuate that mitochondrial oxidative stress was induced ascribable to BA.


Butyric acid Gingival tissue Mitochondria Rat blood Reactive oxygen species homeostasis 



This work was supported by the Dental Research Center-Nihon University School of Dentistry and funded by the Sato Fund-Nihon University School of Dentistry and both the Ministry of Health, Labor and Welfare and the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan through the Strategic Research Base Development Program for Private Universities 2010–2014 (S1001024).


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Copyright information

© Cell Stress Society International 2013

Authors and Affiliations

  • Marni E. Cueno
    • 1
  • Kenichi Imai
    • 1
  • Noriko Matsukawa
    • 2
  • Takamitsu Tsukahara
    • 2
  • Tomoko Kurita-Ochiai
    • 3
  • Kuniyasu Ochiai
    • 1
  1. 1.Department of Microbiology, Division of Immunology and Pathobiology, Dental Research CenterNihon University School of DentistryTokyoJapan
  2. 2.Kyoto Institute of Nutrition and Pathology Inc.KyotoJapan
  3. 3.Department of Microbiology and ImmunologyNihon University School of Dentistry at MatsudoMatsudoJapan

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