Cell Stress and Chaperones

, Volume 18, Issue 4, pp 483–493 | Cite as

Evidence of a role for both anti-Hsp70 antibody and endothelial surface membrane Hsp70 in atherosclerosis

  • Xue Leng
  • Xinxing Wang
  • Wei Pang
  • Rui Zhan
  • Zhiqing Zhang
  • Liqun Wang
  • Xiujie Gao
  • Lingjia QianEmail author
Original Paper


Although previous studies have shown that autoantigens such as Hsps have been implicated by induction of an autoimmune process in the development of atherosclerosis, the exact role of anti-Hsp70 antibody in atherosclerosis is unknown. In the present study, the levels of anti-Hsp70 autoantibodies and oxidized low density lipoprotein (OxLDL) were all significantly increased, and they were strongly correlated in an atherosclerosis model. After the endothelial cells were incubated with 20 μg/mL OxLDL for 12 h at 37 °C and followed by 90 min recovery, Hsp70 positive staining of OxLDL-treated endothelial cells was observed on the cell surface in immunostaining and flow cytometric analysis. This membrane Hsp70 was not from culture supernatant Hsp70 and binding of extracellular Hsp70 but was defined as endothelial surface membrane Hsp70. Furthermore, only in the OxLDL-treated group, but not in the untreated group, 51Cr-labeled endothelial cells were lysed by anti-Hsp70 antibody (BD091, IgAS) in the presence of either complement or peripheral blood mononuclear cells. Control antibodies, including IgNor, mAb to Hsp70 (SPA-810), and mAbs to Factor VIII, α-actin, and CD3 showed no cytotoxic effects. In conclusion, anti-Hsp70 antibodies could be reacting with the endothelial surface membrane Hsp70 induced by OxLDL and were able to mediate endothelial cytotoxicity. There is a possibility that a humoral immune reaction to endothelial surface membrane Hsp70 may play an important role in the pathogenesis of atherosclerosis.


Endothelial cell Surface membrane Hsp70 Anti-Hsp70 antibody OxLDL Atherosclerosis 



Heat shock proteins


Oxidized low density lipoprotein




Atherosclerotic rat plasma Hsp70 autoantibodies


Control rat plasma Hsp70 autoantibodies


Monoclonal antibody


Monoclonal antibody to Hsp70 (which recognized Hsp70 C-terminal)


Monoclonal antibody to Hsp70 (binding site, aa437-504)


Phosphate-buffered saline


Horseradish peroxidase


Tetramethyl benzidine


Enzyme-linked immunosorbent assay



This research was supported by Important National Science & Technology Specific Projects (grant no.2009ZX09103-629) and by the National Natural Science Foundation of China (grant nos. 30800456 and 81000584).

Supplementary material

12192_2013_404_MOESM1_ESM.ppt (153 kb)
ESM 1 (PPT 153 kb)


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Copyright information

© Cell Stress Society International 2013

Authors and Affiliations

  • Xue Leng
    • 2
  • Xinxing Wang
    • 1
  • Wei Pang
    • 3
  • Rui Zhan
    • 1
  • Zhiqing Zhang
    • 3
  • Liqun Wang
    • 3
  • Xiujie Gao
    • 3
  • Lingjia Qian
    • 1
    Email author
  1. 1.Institute of Basic Medical SciencesBeijingPeople’s Republic of China
  2. 2.Institute of Medical EquipmentTianjinPeople’s Republic of China
  3. 3.Institute of Health & Environmental MedicineTianjinPeople’s Republic of China

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