Cell Stress and Chaperones

, Volume 18, Issue 2, pp 193–201 | Cite as

Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects

Original Paper

Abstract

In this paper, two-dimensional flow field simulation was conducted to determine shear stresses and velocity profiles for bone tissue engineering in a rotating wall vessel bioreactor (RWVB). In addition, in vitro three-dimensional fabrication of tissue-engineered bones was carried out in optimized bioreactor conditions, and in vivo implantation using fabricated bones was performed for segmental bone defects of Zelanian rabbits. The distribution of dynamic pressure, total pressure, shear stress, and velocity within the culture chamber was calculated for different scaffold locations. According to the simulation results, the dynamic pressure, velocity, and shear stress around the surface of cell-scaffold construction periodically changed at different locations of the RWVB, which could result in periodical stress stimulation for fabricated tissue constructs. However, overall shear stresses were relatively low, and the fluid velocities were uniform in the bioreactor. Our in vitro experiments showed that the number of cells cultured in the RWVB was five times higher than those cultured in a T-flask. The tissue-engineered bones grew very well in the RWVB. This study demonstrates that stress stimulation in an RWVB can be beneficial for cell/bio-derived bone constructs fabricated in an RWVB, with an application for repairing segmental bone defects.

Keywords

Rotating wall vessel bioreactor FLUENT software Stress stimulation Tissue-engineered bones Animal experiment 

References

  1. Agarwal P (2009) Simulation of heat transfer phenomenon in furnace using fluent-gambit. Thesis, National Institute of Technology, Rourkela.Google Scholar
  2. Collins PC, Miller WM, Papoutsakis ET (1998) Stirred culture of peripheral and cord blood hematopoietic cells offers advantages over traditional static systems for clinically relevant applications. Biotechnol Bioeng 59(5):534–543PubMedCrossRefGoogle Scholar
  3. da Costa Goncalves F, da Rosa Paz AH, Priscila Schmidt L et al (2012) Dynamic culture improves MSC adhesion on freeze-dried bone as a scaffold for bone engineering. World journal of stem cells 4(2):9–16CrossRefGoogle Scholar
  4. Hou Q, Pan HL, Fen QB (2005) Research of optimizing fluid field of agitator by fluent. Mach Des Res 21(3):78–82Google Scholar
  5. Hu JC, Athanasiou KA (2005) Low-density cultures of bovine chondrocytes: effects of scaffold material and culture system. Biomaterials 26(14):2001–2012PubMedCrossRefGoogle Scholar
  6. Liu CX, Abedian R, Meister R et al (2012) Influence of perfusion and compression on the proliferation and differentiation of bone mesenchymal stromal cells seeded on polyurethane scaffolds. Biomaterials 33(4):1052–1064PubMedCrossRefGoogle Scholar
  7. Martin I, Wendt D, Heberer M (2004) The role of bioreactors in tissue engineering. Trends Biotechnol 22(2):80–86PubMedCrossRefGoogle Scholar
  8. Massimo P, Nicola L, Alberto C et al (2004) Modeling of engineered cartilage growth in rotating bioreactors. Chem Eng Sci 59:5035–5040CrossRefGoogle Scholar
  9. Rauh J, Milan F, Günther KP et al (2011) Bioreactor systems for bone tissue engineering. Tissue Eng Part B Rev 17(4):263–280PubMedCrossRefGoogle Scholar
  10. Rodrigues CAV, Fernandes TG, Diogo MM et al (2011) Stem cell cultivation in bioreactors. Biotechnol Adv 29(6):815–829PubMedCrossRefGoogle Scholar
  11. Salter E, Goh B, Hung B et al (2012) Bone tissue engineering bioreactors: a role in the clinic? Tissue Engineering Part B-Reviews 18(1):62–75PubMedCrossRefGoogle Scholar
  12. Song KD, Yang ZM, Liu TQ et al (2006) Fabrication and detection of tissue-engineered bones with bio-derived scaffolds in a rotating bioreactor. Biotechnol Appl Biochem 45:65–74PubMedCrossRefGoogle Scholar
  13. Song KD, Liu TQ, Cui ZF et al (2008) Three-dimensional fabrication of engineered bone with human bio-derived bone scaffolds in a rotating wall vessel bioreactor. J Biomed Mater Res A 86A(2):323–332CrossRefGoogle Scholar
  14. Song AP, Gao S, Wu WW (2010) The mesh characteristics of arch cylindrical gear and its digitization analysis. In: Proceedings of the 2010 International Conference on Digital Manufacturing & Automation, vol 2, pp 720–724Google Scholar
  15. Song KD, Liu Y, Wang H et al (2011) Ex vivo expansion of human umbilical cord blood hematopoietic stem/progenitor cells with support of microencapsulated rabbit mesenchymal stem cells in a rotating bioreactor. Tissue Engineering and Regenerative Medicine 8(3):334–345Google Scholar
  16. Van Dyke WS, Sun XH, Richard AB et al (2012) Novel mechanical bioreactor for concomitant fluid shear stress and substrate strain. J Biomech 45(7):1323–1327PubMedCrossRefGoogle Scholar
  17. Volkmer E, Drosse I, Otto S et al (2008) Hypoxia in static and dynamic 3D culture systems for tissue engineering of bone. Tissue Eng Part A 14(8):1331–1340PubMedCrossRefGoogle Scholar
  18. Wang D, Liu W, Han B et al (2005) The bioreactor: a powerful tool for large-scale culture of animal cells. Curr Pharm Biotechnol 6(5):397–403PubMedCrossRefGoogle Scholar
  19. Yeatts AB, Fisher JP (2011) Bone tissue engineering bioreactors: dynamic culture and the influence of shear stress. Bone 48:171–181PubMedCrossRefGoogle Scholar
  20. Yu XJ, Botchwey EA, Levine EM et al (2004) Bioreactor-based bone tissue engineering: the influence of dynamic flow on osteoblast phenotypic expression and matrix mineralization. Pans 101:11203–11208CrossRefGoogle Scholar
  21. Zhu YX, Liu TQ, Ye H et al (2010) Enhancement of adipose-derived stem cell differentiation in scaffolds with IGF-I gene impregnation under dynamic microenvironment. Stem cells and development 19(10):1547–1556PubMedCrossRefGoogle Scholar

Copyright information

© Cell Stress Society International 2012

Authors and Affiliations

  1. 1.Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine ChemicalsDalian University of TechnologyDalianChina
  2. 2.Division of Bioengineering, School of Chemical and Biomedical EngineeringNanyang Technological UniversitySingaporeSingapore

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