Cell Stress and Chaperones

, Volume 18, Issue 2, pp 193–201 | Cite as

Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects

Original Paper


In this paper, two-dimensional flow field simulation was conducted to determine shear stresses and velocity profiles for bone tissue engineering in a rotating wall vessel bioreactor (RWVB). In addition, in vitro three-dimensional fabrication of tissue-engineered bones was carried out in optimized bioreactor conditions, and in vivo implantation using fabricated bones was performed for segmental bone defects of Zelanian rabbits. The distribution of dynamic pressure, total pressure, shear stress, and velocity within the culture chamber was calculated for different scaffold locations. According to the simulation results, the dynamic pressure, velocity, and shear stress around the surface of cell-scaffold construction periodically changed at different locations of the RWVB, which could result in periodical stress stimulation for fabricated tissue constructs. However, overall shear stresses were relatively low, and the fluid velocities were uniform in the bioreactor. Our in vitro experiments showed that the number of cells cultured in the RWVB was five times higher than those cultured in a T-flask. The tissue-engineered bones grew very well in the RWVB. This study demonstrates that stress stimulation in an RWVB can be beneficial for cell/bio-derived bone constructs fabricated in an RWVB, with an application for repairing segmental bone defects.


Rotating wall vessel bioreactor FLUENT software Stress stimulation Tissue-engineered bones Animal experiment 



This work was supported by the Fok Ying Tung Education Foundation (132027), the National Science Foundation of China (31170945/30700181), the Fundamental Research Funds for the Central Universities (DUT11SM09/DUT12JB09), and the SRF for ROCS, SEM.


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Copyright information

© Cell Stress Society International 2012

Authors and Affiliations

  1. 1.Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine ChemicalsDalian University of TechnologyDalianChina
  2. 2.Division of Bioengineering, School of Chemical and Biomedical EngineeringNanyang Technological UniversitySingaporeSingapore

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