Cell Stress and Chaperones

, Volume 17, Issue 4, pp 435–444

Stress levels over time in the introduced ascidian Styela plicata: the effects of temperature and salinity variations on hsp70 gene expression

  • Mari Carmen Pineda
  • Xavier Turon
  • Susanna López-Legentil
Original Paper

DOI: 10.1007/s12192-012-0321-y

Cite this article as:
Pineda, M.C., Turon, X. & López-Legentil, S. Cell Stress and Chaperones (2012) 17: 435. doi:10.1007/s12192-012-0321-y

Abstract

Species distribution, abundance, and long-term survival are determined by biotic and abiotic regimes. However, little is known about the importance of these factors in species range expansion. Styela plicata is a solitary ascidian introduced all over the world by ship fouling, including salt marsh habitats, where introduced populations must tolerate high seasonal variations in temperature and salinity. To determine the seasonal stress levels in a salt marsh population of S. plicata, we quantified heat shock protein (hsp70) gene expression using quantitative real-time PCR throughout a 2-year cycle. Results showed that hsp70 expression varied over time, with higher stress levels recorded in summer and winter. Periodic conditions of high temperatures, particularly when coupled with low salinities, increased hsp70 gene expression. Mortality events observed every year around June were concurrent with sharp increases in temperature (>6°C), indicating that drastic changes in abiotic factors may overwhelm the observed stress response mechanisms. Determining the ability of introduced species to cope with stress, and the thresholds above which these mechanisms fail, is fundamental to predict the potential expansion range of introduced species and design efficient containment plans.

Keywords

Hsp70 Salinity Temperature Introduced species Ascidian Salt marsh 

Copyright information

© Cell Stress Society International 2012

Authors and Affiliations

  • Mari Carmen Pineda
    • 1
  • Xavier Turon
    • 2
  • Susanna López-Legentil
    • 1
  1. 1.Department of Animal BiologyUniversity of BarcelonaBarcelonaSpain
  2. 2.Center for Advanced Studies of Blanes (CEAB-CSIC)BlanesSpain

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