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Cell Stress and Chaperones

, Volume 16, Issue 3, pp 287–296 | Cite as

Lipopolysaccharide pretreatment protects against ischemia/reperfusion injury via increase of HSP70 and inhibition of NF-κB

  • Yong-wei Yao
  • Guo-hui Zhang
  • Ying-yu Zhang
  • Wei-dong Li
  • Cheng-hua Wang
  • Chun-yang Yin
  • Fu-min Zhang
Original Paper

Abstract

It has been reported that pretreatment of rats with lipopolysaccharide (LPS) increases myocardial functional recovery in ischemia/reperfusion (I/R) hearts. However, the mechanisms by which LPS induces cardioprotection against I/R injury have not been fully elucidated. In this study, we pretreated rats with LPS (1.0 mg/kg) 24 h before they were subjected to I/R injury, and then examined the roles of heat shock protein-70 (HSP70) and nucleus factor-κB (NF-κB) in LPS-induced cardioprotection. We observed that pretreatment with low-dose LPS resulted in significantly increased levels of HSP70 in the myocardium, which could dramatically inhibit NF-κB translocation and reduce degradation of inhibitory κB. Inhibition of NF-κB, in turn, attenuated release of inflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-1β, and IL-6) and reduced apoptosis of myocardium and infarct area following I/R injury. Moreover, HSP70 could ameliorate oxidative stress following I/R injury. To further investigate whether increase of HSP70 might be responsible for protection of the myocardium against I/R injury, we co-administered the HSP70 inhibitor, quercetin, with LPS before I/R injury. We found that LPS-induced cardioprotection was attenuated by co-administration with quercetin. Herein, we concluded that increased levels of HSP70 through LPS pretreatment led to inhibition of NF-κB activity in the myocardium after I/R injury. Our results indicated that LPS-induced cardioprotection was mediated partly through inhibition of NF-κB via increase of HSP70, and LPS pretreatment could provide a means of reducing myocardial I/R injury.

Keywords

Lipopolysaccharide Heat shock protein 70 NF-κB Ischemia/reperfusion injury 

Notes

Acknowledgments

This project was supported by grants from the Technology Bureau Foundation of Zhenjiang City, Jiangsu Province (No. SH2008043).

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Copyright information

© Cell Stress Society International 2010

Authors and Affiliations

  • Yong-wei Yao
    • 1
  • Guo-hui Zhang
    • 1
  • Ying-yu Zhang
    • 1
  • Wei-dong Li
    • 1
  • Cheng-hua Wang
    • 1
  • Chun-yang Yin
    • 1
  • Fu-min Zhang
    • 2
  1. 1.Department of CardiologyAffiliated People’s Hospital of Jiangsu UniversityZhenjiangPeople’s Republic of China
  2. 2.Department of CardiologyFirst Affiliated Hospital of Nanjing Medical UniversityNanjingPeople’s Republic of China

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