Cell Stress and Chaperones

, Volume 15, Issue 5, pp 717–727 | Cite as

A single dose of carbon monoxide intraperitoneal administration protects rat intestine from injury induced by lipopolysaccharide

Original Paper
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Abstract

Treatment with inhaled carbon monoxide (CO) has been shown to ameliorate intestinal injury induced by lipopolysaccharide (LPS) or ischemia-reperfusion in experimental animals. We hypothesized that CO intraperitoneal administration (i.p) might provide similar protection against inhaled gas. In the present study, 1 h after intravenously receiving 5 mg/kg LPS, rats were exposed to either room air or 2 ml/kg of 250 ppm CO i.p for 1, 3, and 6 h. Intestinal tissues were collected to determine the levels of platelet activator factor (PAF), intercellular adhesion molecule-1 (ICAM-1), interlukin-10 (IL-10), maleic dialdehyde (MDA), cell apoptotic rate and the phosphorylated p38 mitogen activated protein kinase (MAPK), as well as myeloperoxidase (MPO) and superoxide dismutase (SOD) activity. After CO i.p, the increase of PAF, ICAM-1, MDA, MPO, and cell apoptosis rate induced by LPS was markedly reduced (P < 0.05 or 0.01), while the decrease of IL-10 and SOD was significantly increased (P < 0.05). Western blotting showed that the effects of CO i.p were mediated by p38 MAPK pathway. Thus, the results of our study show that CO i.p exerts potent protection against LPS induced injury to the intestine via anti-oxidant, anti-inflammation and anti-apoptosis, which may involve the p38 MAPK pathway.

Keywords

Carbon monoxide Intestine Injury Mitogen-activated protein kinase 
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Notes

Acknowledgments

This work is supported by the grants from the Natural Science Foundation of Jiangsu Province (No. 98029) and the Scientific Development Foundation of Jiangsu Province (No. 2000409). We thank Professor LI Chun-Sheng for assistance with the experiments, Professor XU Jian-Guo for valuable discussions, and Dr ZHANG Xiao-Liang for statistical analyses.

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Copyright information

© Cell Stress Society International 2010

Authors and Affiliations

  1. 1.Intensive Care Unit, The First Affiliated HospitalNanjing Medical UniversityNanjingChina
  2. 2.Emergency Department, Huashan HospitalFudan UniversityShanghaiChina
  3. 3.Emergency Department, The Sixth HospitalShanghai Jiaotong UniversityShanghaiChina
  4. 4.NanjingChina

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