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Cell Stress and Chaperones

, Volume 15, Issue 5, pp 545–553 | Cite as

Heteromeric complexes of heat shock protein 70 (HSP70) family members, including Hsp70B′, in differentiated human neuronal cells

  • Ari M. Chow
  • Philip Mok
  • Dawn Xiao
  • Sam Khalouei
  • Ian R. BrownEmail author
Original Paper

Abstract

Human neurodegenerative disorders such as Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis have been termed “protein misfolding disorders.” Upregulation of heat shock proteins that target misfolded aggregation-prone proteins has been proposed as a potential therapeutic strategy to counter neurodegenerative disorders. The heat shock protein 70 (HSP70) family is well characterized for its cytoprotective effects against cell death and has been implicated in neuroprotection by overexpression studies. HSP70 family members exhibit sequence and structural conservation. The significance of the multiplicity of HSP70 proteins is unknown. In this study, coimmunoprecipitation was employed to determine if association of HSP70 family members occurs, including Hsp70B′ which is present in the human genome but not in mouse and rat. Heteromeric complexes of Hsp70B′, Hsp70, and Hsc70 were detected in differentiated human SH-SY5Y neuronal cells. Hsp70B′ also formed complexes with Hsp40 suggesting a common co-chaperone for HSP70 family members.

Keywords

SH-SY5Y neuronal cells Hsp70B′ Hsp70 Hsc70 Heteromeric complexes Celastrol 

Notes

Acknowledgments

We thank Erin Chang, Abelyn K. Lim, and Nasim M. Zamir for technical assistance. This study was supported by grants to I.R.B. from National Science and Engineering Research Council of Canada.

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Copyright information

© Cell Stress Society International 2010

Authors and Affiliations

  • Ari M. Chow
    • 1
  • Philip Mok
    • 1
  • Dawn Xiao
    • 1
  • Sam Khalouei
    • 1
  • Ian R. Brown
    • 1
    Email author
  1. 1.Centre for the Neurobiology of StressUniversity of Toronto ScarboroughTorontoCanada

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