Abstract
Hsp90 is an essential eukaryotic molecular chaperone that stabilizes a large set of client proteins, many of which are involved in various cellular signaling pathways. The current list of Hsp90 interactors comprises about 200 proteins and this number is growing steadily. In this paper, we report on the application of three complementary proteomic approaches directed towards identification of novel proteins that interact with Hsp90. These methods are coimmunoprecipitation, pull down with biotinylated geldanamycin, and immobilization of Hsp90β on sepharose. In all, this study led to the identification of 42 proteins, including 18 proteins that had not been previously characterized as Hsp90 interactors. These novel Hsp90 partners not only represent abundant protein species, but several proteins were identified at low levels, among which signaling kinase Cdk3 and putative transcription factor tripartite motif-containing protein 29. Identification of tetratricopeptide-repeat-containing mitochondrial import receptor protein Tom34 suggests the involvement of Hsp90 in the early steps of translocation of mitochondrial preproteins. Taken together, our data expand the knowledge of the Hsp90 interactome and provide a further step in our understanding of the Hsp90 chaperone system.
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Abbreviations
- ACN:
-
acetonitrile
- Cdk3:
-
cyclin-dependent kinase 3
- CNBr:
-
cyanogen bromide
- DSP:
-
Dithiobis[succinimidyl propionate]
- GA:
-
geldanamycin
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Acknowledgments
S.R. was supported by a Marie-Curie-Excellent Grant of the EU, a VIDI grant of the Netherlands Organization for Science (NWO), and a High Potential Grant of Utrecht University. The authors thank the Netherlands Proteomics Center for financial support. The authors thank Dimitrios Argyropoulos for his assistance in experimental work.
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This paper reports on identification of 18 novel Hsp90 partners, including signaling kinase Cdk3, putative transcription factor TRIM29, and Tom34. Presented data expand the knowledge of the Hsp90 interactome and provide a further step in our understanding of the Hsp90 chaperone system.
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Tsaytler, P.A., Krijgsveld, J., Goerdayal, S.S. et al. Novel Hsp90 partners discovered using complementary proteomic approaches. Cell Stress and Chaperones 14, 629–638 (2009). https://doi.org/10.1007/s12192-009-0115-z
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DOI: https://doi.org/10.1007/s12192-009-0115-z