Skip to main content

Advertisement

SpringerLink
Log in

Relationship between donor-specific HPA-15 antibodies and poor graft function in HPA-15 mismatched cord blood transplantation

  • Case Report
  • Published:
International Journal of Hematology Aims and scope Submit manuscript

Abstract

Poor graft function (PGF) is a fatal complication following hematopoietic stem cell transplantation and is influenced by multiple factors, such as donor-specific anti-HLA antibodies, a poor infused CD34+ cell count, and the donor source. Alloantibodies against human platelet antigen 15 (HPA-15) recognize platelet membrane glycoprotein CD109, which is expressed not only on platelets, but also on megakaryocytes and specific hematopoietic stem cells. HPA-15 antibodies are known to induce platelet transfusion refractoriness and neonatal alloimmune thrombocytopenia, but their effects on graft function following hematopoietic stem cell transplantation remain unknown. We encountered a case of HPA-15 mismatched cord blood transplantation with a high HPA-15b antibody titer. Prolonged PGF and megakaryocyte aplasia with sustained high-titer HPA-15b antibodies were attenuated by rituximab therapy, and rapid recovery of hematopoiesis was achieved. HPA-15-compatible platelet transfusions were highly effective for platelet recovery. Methylcellulose assays and megakaryocyte cultures revealed that patient serum inhibited in vitro hematopoietic development from patient bone marrow cells. These results suggest that HPA-15 antibodies might be a cause of PGF and that reducing the HPA-15 antibody titer might improve graft function in HPA-15 mismatched transplantation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Chen J, Wang H, Zhou J, Feng S. Advances in the understanding of poor graft function following allogeneic hematopoietic stem-cell transplantation. Ther Adv Hematol. 2020;11:1–13.

    Google Scholar 

  2. Zhao Y, Gao F, Shi J, Luo Y, Tan Y, Lai X, et al. Incidence, risk factors, and outcomes of primary poor graft function after allogeneic hematopoietic stem cell transplantation. Biol Blood Marrow Transplant. 2019;25:1898–907.

    Article  Google Scholar 

  3. Hayashi T, Hirayama F. Advances in alloimmune thrombocytopenia: perspectives on current concepts of human platelet antigens, antibody detection strategies, and genotyping. Blood Transfus. 2015;13(3):380–90.

    PubMed  PubMed Central  Google Scholar 

  4. Schuh AC, Watkins NA, Nguyen Q, Harmer NJ, Lin M, Prosper JY, et al. A tyrosine703serine polymorphism of CD109 defines the Gov platelet alloantigens. Blood. 2002;99:1692–8.

    Article  CAS  Google Scholar 

  5. Murray LJ, Bruno E, Uchida N, Hoffman R, Nayar R, Yeo EL, et al. CD109 is expressed on a subpopulation of CD34+ cells enriched in hematopoietic stem and progenitor cells. Exp Hematol. 1999;27:1282–94.

    Article  CAS  Google Scholar 

  6. Kanda Y. Investigation of the freely available easy-to-use software ‘EZR’ for medical statistics. Bone Marrow Transplant. 2013;48:452–8.

  7. Lewin A, Al Khan S, Beaudin L, Meilleur L, Clarke G, Richard L. Report on the 19th International Society of Blood Transfusion Platelet Immunology Workshop 2018. Vox Sang. 2020;115:767–82.

    Article  CAS  Google Scholar 

  8. Hayashi T, Amakishi E, Matsuyama N, Yasui K, Furuta RA, Hori Y, et al. Detection of antibodies against human platelet antigens 15a and 15b by using a cell line panel. Br J Haematol. 2010;151:402–4.

    Article  Google Scholar 

  9. Inoue H, Sakamoto R, Nishimiya H, Sakamoto H, Terasu S, Aminaka R, et al. Minor impact of patient alloantibodies against human platelet antigen (HPA)-15 in the effectiveness of platelet transfusion: a pilot study. Transfusion. 2021;61:738–43.

    Article  CAS  Google Scholar 

  10. Matsuhashi M, Tsuno NH, Sone S, Mishima Y, Nagura Y, Watanabe-Okochi N, et al. The role of alloantibodies against human platelet antigen-15 in multiply platelet transfused patients. Transfusion. 2014;54:1093–9.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Hematology, Rinku General Medical Center, 2-3 Ourai-Kita, Rinku, Izumisano, Osaka, 598-8577, Japan

    Masato Yasumi, Takaya Endo, Shinsuke Kusakabe & Takahiro Karasuno

  2. Japanese Red Cross Kinki Block Blood Center, Ibaraki, Japan

    Yangsook Koh, Hiroe Sakamoto & Hiroko Inoue

  3. Department of Hematology and Oncology, Osaka University Graduate School of Medicine, Osaka, Japan

    Takafumi Yokota, Shinsuke Kusakabe, Takao Sudo & Naoki Hosen

Authors
  1. Masato Yasumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takafumi Yokota
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takaya Endo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shinsuke Kusakabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yangsook Koh
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hiroe Sakamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hiroko Inoue
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Takao Sudo
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Naoki Hosen
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Takahiro Karasuno
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masato Yasumi.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yasumi, M., Yokota, T., Endo, T. et al. Relationship between donor-specific HPA-15 antibodies and poor graft function in HPA-15 mismatched cord blood transplantation. Int J Hematol 115, 753–758 (2022). https://doi.org/10.1007/s12185-022-03286-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12185-022-03286-z

Keywords

Search

Navigation