Expression of activated molecules on CD5+B lymphocytes in autoimmune hemolytic anemia
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To investigate the expression of activation molecules on CD5+B lymphocytes in peripheral blood of autoimmune hemolytic anemia (AIHA)/Evans patients. The expression of CD80, CD86, and CD69 on CD5+B lymphocytes was detected using flow cytometry in 30 AIHA/Evans patients, 18 normal controls (NC) and nine chronic lymphocytic leukemia (CLL) patients. CD80 on CD5+B lymphocytes in untreated patients was higher than that in remission patients (P < 0.05), NC (P < 0.01) and CLL patients (P < 0.01). CD80 on CD5+B lymphocytes was higher than that on CD5−B lymphocytes in untreated patients (P > 0.05), but lower than those of CD5−B lymphocytes in remission patients and NC (P < 0.05). CD86 on CD5+B lymphocytes of untreated patients was higher than that of remission patients (P < 0.05), NC (P < 0.01). CD86 on CD5+B lymphocytes of CLL was higher than that of NC, remission (P < 0.05), and untreated patients (P > 0.05). CD80 and CD86 on CD5+B lymphocytes was negatively correlated with hemoglobin (HB), C3, C4 (P < 0.05) and positively correlated with reticulocyte (Ret) (P < 0.05). CD69 on CD5+ and CD5−B lymphocytes of CLL was higher than those of AIHA/Evans patients and NC (P < 0.05). The active molecules on CD5+B lymphocytes in peripheral blood of AIHA/Evans patients differ from those on CD5− and clonal CD5+B lymphocytes.
KeywordsAutoimmune hemolytic anemia CD5+B lymphocytes CD80 CD86 CD69
This work was supported by grants from application foundation and advanced technology research project of Tianjin (15JCYBJC27300), National Natural Science Foundation of China (81370607), Science and Technology Foundation of Tianjin Health Bureau (09KZ108).
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Conflict of interest
The authors declare that they have no conflict of interest.
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