Prediction model for CD34 positive cell yield in peripheral blood stem cell collection on the fourth day after G-CSF administration in healthy donors
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Allogeneic peripheral blood stem cell transplantation (PBSCT) is an indispensable treatment option for hematological malignancy. The optimal collection day after granulocyte colony-stimulating factor (G-CSF) administration should be determined by peripheral blood pre-apheresis CD34 positive (CD34+) cell percentage. However, pre-apheresis CD34+ cell analysis is not available for most institutions in Japan. Prediction of the optimal collection day based on objective parameters, other than direct CD34+ cell count, is thus an important matter for investigation. To identify potential predictive factors, clinical parameters in 79 related donors who received allogeneic peripheral blood stem cell (PBSC) collection were analyzed. Eight factors were significantly correlated with the number of CD34+ cells per donor body weight on the fourth day (day 4) after G-CSF administration in univariate analysis. Using multi-regression analysis, we made a simple scoring system comprising age, sex, LDH on day 4 and RBC count at the baseline, which significantly predicted CD34+ cell yield (P = 0.048). This system allows us to determine the optimal PBSC collection day. When the score is 0 or 1 on day 4, starting apheresis on day 5 potentially helps avoiding the need for multiple harvests. Score 3 or 4 on day 4 is indicative of better performance if apheresis is started on day 4.
KeywordsPeripheral blood stem cell transplantation Donor CD34+ cells Apheresis
We are grateful for the technical assistance of all the staff of the Department of Transfusion Medicine and the Department of Clinical Laboratory, in especial to Ms. Mika Matsuhashi, Ms. Yuuko Mishima, Mr. Masahiro Johna, and Mr. Naoyuki Yoshikawa.
Conflict of interest
The authors declare that they have no conflicts of interest relevant to the manuscript.
- 6.Tigue CC, McKoy JM, Evens AM, Trifilio SM, Tallman MS, Bennett CL. Granulocyte-colony stimulating factor administration to healthy individuals and persons with chronic neutropenia or cancer: an overview of safety considerations from the Research on Adverse Drug Events and Reports project. Bone Marrow Transplant. 2007;40:185–92.PubMedCrossRefGoogle Scholar
- 7.Stroncek DF, Clay ME, Petzoldt ML, Smith J, Jaszcz W, Oldham FB, et al. Treatment of normal individuals with granulocyte-colony-stimulating factor: donor experiences and the effects on peripheral blood CD34+ cell counts and on the collection of peripheral blood stem cells. Transfusion. 1996;36:601–10.PubMedCrossRefGoogle Scholar
- 11.Sekhsaria S, Fleisher TA, Vowells S, Brown M, Miller J, Gordon I, et al. Granulocyte colony-stimulating factor recruitment of CD34+ progenitors to peripheral blood: impaired mobilization in chronic granulomatous disease and adenosine deaminase—deficient severe combined immunodeficiency disease patients. Blood. 1996;88:1104–12.PubMedGoogle Scholar
- 13.Guideline for allogeneic peripheral blood stem cell collection from unrelated donors. 2010 [cited 2013 Feb]. http://www.jmdp.or.jp/documents/file/04_medical/f-up03a.pdf.
- 17.Anguita-Compagnon AT, Dibarrart MT, Palma J, Paredes L, Mosso C, Montalva R, et al. Mobilization and collection of peripheral blood stem cells: guidelines for blood volume to process, based on CD34-positive blood cell count in adults and children. Transplant Proc. 2010;42:339–44.PubMedCrossRefGoogle Scholar
- 19.Cytometry, S.o.F. Report of the Hematology Standardization Committee Guidelines for CD34+ Cell Determination by Flow Cytometry (JCCLS H3-P V1.0). 2007 [cited 2013 20th, February]; http://www.jccls.org/techreport/fct_h3pv1.pdf.
- 21.Passos-Coelho JL, Braine HG, Davis JM, Huelskamp AM, Schepers KG, Ohly K, et al. Predictive factors for peripheral-blood progenitor-cell collections using a single large-volume leukapheresis after cyclophosphamide and granulocyte-macrophage colony-stimulating factor mobilization. J Clin Oncol. 1995;13:705–14.PubMedGoogle Scholar
- 22.Elliott C, Samson DM, Armitage S, Lyttelton MP, McGuigan D, Hargreaves R, et al. When to harvest peripheral-blood stem cells after mobilization therapy: prediction of CD34-positive cell yield by preceding day CD34-positive concentration in peripheral blood. J Clin Oncol. 1996;14:970–3.PubMedGoogle Scholar