Quality assessment of umbilical cord blood units at the time of transplantation

  • Yoshinori Kudo
  • Masayoshi Minegishi
  • Osamu Seki
  • Hiroyuki Takahashi
  • Akira Suzuki
  • Ayuko Narita
  • Yuko Sato
  • Machiko Abe
  • Natsuko Ishioka
  • Hideo Harigae
  • Shigeru Tsuchiya
Original Article

DOI: 10.1007/s12185-011-0830-y

Cite this article as:
Kudo, Y., Minegishi, M., Seki, O. et al. Int J Hematol (2011) 93: 645. doi:10.1007/s12185-011-0830-y

Abstract

Total nucleated cell (TNC) count, CD34+ cell count, colony-forming unit-granulocyte–macrophage (CFU-GM) content, and cell viability impact the outcome of umbilical cord blood (UCB) transplantation. Assessments of unit quality have usually been provided by cord blood banks (CBBs), but it is unclear whether pre-freezing tests or pre-transplant release tests performed by CBBs are reproducible. The aim of this study was to compare the UCB characteristics analyzed at the site of infusion of the UCB with those provided by CBBs. Samples were taken from 54 UCB units for assessment of post-thaw characteristics. TNC counts and CD34+ cell contents measured at our hospital before infusion showed good correlations with values assessed in pre-freezing tests (r = 0.900 and 0.943, respectively) and pre-transplant release tests (r = 0.829 and 0.930, respectively). Our data reveal that the TNC counts and CD34+ cell contents determined by pre-freezing and pre-transplant release tests, which are the most important UCB unit selection criteria, accurately reflected the quality of infused UCB units. However, CFU-GM content was poorly correlated (r = 0.560 and 0.606). Correlation of post-thaw cell viabilities measured before infusion and during the pre-transplant release tests was also poor (r = 0.308). We suggest that the TNC count and CD34+ cell content estimated before cryopreservation and in pre-transplant release tests provided by CBBs are reproducible and can assist the transplant physicians in selection of appropriate UCB units.

Keywords

Hematopoietic stem cell transplantation Umbilical cord blood Cord blood bank Cryopreservation Quality 

Copyright information

© The Japanese Society of Hematology 2011

Authors and Affiliations

  • Yoshinori Kudo
    • 1
  • Masayoshi Minegishi
    • 1
  • Osamu Seki
    • 1
  • Hiroyuki Takahashi
    • 1
  • Akira Suzuki
    • 1
  • Ayuko Narita
    • 1
  • Yuko Sato
    • 1
  • Machiko Abe
    • 1
  • Natsuko Ishioka
    • 1
  • Hideo Harigae
    • 2
  • Shigeru Tsuchiya
    • 3
  1. 1.Division of Blood TransfusionTohoku University HospitalSendaiJapan
  2. 2.Department of Rheumatology and HematologyTohoku University Graduate School of MedicineSendaiJapan
  3. 3.Department of PediatricsTohoku University Graduate School of MedicineSendaiJapan

Personalised recommendations