Immunohistochemical study association between human herpesvirus 8 and multiple myeloma

  • Mohammad Hadi Sadeghian
  • Mehrdad Katebi
  • Hossein Ayatollahi
  • Mohammad Reza Keramati
Original Article

Abstract

Multiple myeloma (MM) is a plasma cell neoplasm characterized by a clonal proliferation of plasma cells that produce a monoclonal protein and involve the skeleton at multiple sites. Although human herpesvirus-8(HHV-8) has been implicated in pathogenesis of disease, but this role is not clear. The aim of this study was to show direct evidence of HHV-8 in bone marrow tissue of MM patients with use of immunohistochemical method. Standard immunohistochemstry was performed with HHV-8 marker on formalin fixed paraffin embedded bone marrow tissue in 30 MM cases and 30 subjects with normal bone marrow tissue. Slides were examined for nuclear immunoreactivity by two pathologists. The data were analyzed with 2 × 2 contingency tables and Fischer’s exact test, also differences with P-value under 0.05 (P ≤ 0.05) was considered statistically significant. Nuclear immunoreactivity for HHV-8 was detected in four patients (13.3%) with MM but was not detectable in any normal bone marrow cells. Fisher exact test showed no difference for HHV-8 immunoreactivity between two groups of case and control (P = 0.11). Our finding demonstrated that HHV-8 infection did not seem influence on pathogenesis of multiple myeloma.

Keywords

Multiple myeloma Herpesvirus-8 Immunohistochemistry Bone marrow 

References

  1. 1.
    Brunning RD. Bone marrow. In: Rosai J, editor. Rosai and Ackerman’s surgical pathology. 9th edn. Edinburgh:Mosby;2004. p. 2100.Google Scholar
  2. 2.
    Requena L, Kutzner H, Palmedo G, Calonje E, Requena C, Pérez G, et al. Cutaneous involvement in multiple myeloma: a clinicopathologic, immunohistochemical, and cytogenetic study of 8 cases. Arch Dermatol. 2003;139(4):475–86.PubMedCrossRefGoogle Scholar
  3. 3.
    Lari MM, Abtahi M, Hosseini RF. 33 cases of multiple myeloma with massive bone destruction. Report of a 10-year study in northeastern Iran. Orthop Rev. 1986;15(1):52–5.PubMedGoogle Scholar
  4. 4.
    Aster JC. Disease of white blood cells, lymph nodes, spleen, thymus. In: Kumar V, Abbas AK, Fausto N, editors. Robbins and Cotran pathologic basis of disease. 7th ed. Pennsylvania: Elsevier; 2005. p. 679.Google Scholar
  5. 5.
    Csire M, Mikala G, Peto M, Jánosi J, Juhász A, Tordai A, et al. Detection of four lymphotropic herpesviruses in Hungarian patients with multiple myeloma and lymphoma. FEMS Immunol Med Microbiol. 2007;49(1):62–7.PubMedCrossRefGoogle Scholar
  6. 6.
    Ancín I, Sarrá J, Peris J, Romagosa V, Domingo-Claros A, Grañena A. Demonstration of Epstein-Barr virus in a case of multiple myeloma after renal transplantation. Haematologica. 2000;85(7):773–4.PubMedGoogle Scholar
  7. 7.
    Sarid R, Klepfish A, Schattner A. Virology, pathogenetic mechanisms, and associated diseases of Kaposi sarcoma-associated herpesvirus (human herpesvirus 8). Mayo Clin Proc. 2002;77(9):941–9.PubMedCrossRefGoogle Scholar
  8. 8.
    Santón Roldán A, De San José S, Gómez Sanz E, Fernández Muñoz R, Herrera P, Bellas Menéndez C. Human herpesvirus–8 detection in Kaposi’s sarcoma, multiple myeloma, and lymphoproliferative syndromes occurring in immunocompetent and immunocompromised patients. Med Clin (Barc). 2002;119(7):241–4.Google Scholar
  9. 9.
    Malnati MS, Dagna L, Ponzoni M, Lusso P. Human herpesvirus 8 (HHV–8/KSHV) and hematologic malignancies. Rev Clin Exp Hematol. 2003;7(4):375–405.PubMedGoogle Scholar
  10. 10.
    Dong Y, Zhu P, Ma M. Quantitative analysis of human herpesvirus type 8 and expression of its genes in patients with multiple myeloma. Zhonghua Yi Xue Za Zhi. 2001;81(20):1230–3.PubMedGoogle Scholar
  11. 11.
    Ma HJ, Sjak-Shie NN, Vescio RA, Kaminsky M, Mikail A, Pold M, et al. Human herpesvirus 8 open reading frame 26 and open reading frame 65 sequences from multiple myeloma patients: a shared pattern not found in Kaposi’s sarcoma or primary effusion lymphoma. Clin Cancer Res. 2000;6:4226–33.PubMedGoogle Scholar
  12. 12.
    Fonseca R, Barlogie B, Bataille R, Bastard C, Bergsagel PL, Chesi M, et al. Genetics and cytogenetics of multiple myeloma: A workshop report. Cancer Res. 2004;64:1546–58.PubMedCrossRefGoogle Scholar
  13. 13.
    Tedeschi R, Luostarinen T, De Paoli P, Gislefoss RE, Tenkanen L, Virtamo J, et al. Joint Nordic prospective study on human herpesvirus 8 and multiple myeloma risk. Br J Cancer. 2005;93(7):834–7.PubMedCrossRefGoogle Scholar
  14. 14.
    Regamey N, Hess V, Passweg J, Hess C, Steiger J, Erb P, et al. Infection with human herpesvirus 8 and transplant-associated gammopathy. Transplantation. 2004;77(10):1551–4.PubMedCrossRefGoogle Scholar
  15. 15.
    Tsai WH, Lee YM, Ing-Tiau Kuo B, Ho CK, Liao PT, Liu MD, et al. Increased seroprevalence of human herpesvirus 8 in patients with hematological disorders. Acta Haematol. 2005;114(2):95–8.PubMedCrossRefGoogle Scholar
  16. 16.
    Pan L, Milligan L, Michaeli J, Cesarman E, Knowles DM. Polymerase chain reaction detection of Kaposi’s sarcoma-associated herpesvirus-optimized protocols and their application to myeloma. J Mol Diagn. 2001;3(1):32–8.PubMedGoogle Scholar
  17. 17.
    Saylam G, Yücel OT, Sungur A, Onerci M. Proliferation, angiogenesis and hormonal markers in juvenile nasopharyngeal angiofibroma. Int J Pediatr Otorhinolaryngol. 2006;70(2):227–34.PubMedCrossRefGoogle Scholar
  18. 18.
    Dispenzieri A, Lacy MQ, Greipp PR. Multiple myeloma. In: Greer JP, Foerster J, Lukens J, Rodgers GM, Paraskevas F, Glader B, editors. Wintrob’s clinical hematology. 11th edn ed. Philadelphia: Lippincott; 2004. p. 2584–5.Google Scholar
  19. 19.
    Cancer control office in Center for disease control. Iranian annual cancer registrian report 2003. March 2005(1383). p115.Google Scholar
  20. 20.
    Goedert JJ. The epidemiology of acquired immunodefidency syndrome malignancies. Semin Oncol. 2000;27:390–401.PubMedGoogle Scholar
  21. 21.
    Montella M, Crispo A, Frigeri F, Ronga D, Tridente V, De Marco M, et al. HCV and tumors correlated with immune system: a case-control study in an area of hyperendemicity. Lcuk Res. 2001;25:775–81.CrossRefGoogle Scholar
  22. 22.
    An J, Lichtenstein AX, Brent G, Rettig MB. The Kaposi sarcoma-associated herpesvirus (KSHV) induces cellular interleukin 6 expression: role of the KSHV latency-associated nuclear antigen and the AP1 response element. Blood. 2002;99(2):649–54.PubMedCrossRefGoogle Scholar
  23. 23.
    Tedeschi R, Kvarnung M, Knekt P, Schulz TF, Szekely L, De Paoli PD, et al. A prospective Seroepidemiological study of human herpesvirus 8 infection and the risk of multiple myeloma. Br J Cancer. 2001;84(1):122–5.PubMedCrossRefGoogle Scholar
  24. 24.
    Sun CM, Nae LS. Questionable role of human herpesvirus-8 in the pathogenesis of multiple myeloma. Korean J Pathol. 2005;39(3):164–7.Google Scholar
  25. 25.
    Beksac M, Ma M, Akyerli C, Der Danielian M, Zhang L, Liu J, et al. Frequent demonstration of human herpesvirus 8 (HHV–8) in bone marrow biopsy samples from Turkish patients with multiple myeloma (MM). Leukemia. 2001;15(8):1268–73.PubMedCrossRefGoogle Scholar
  26. 26.
    Rettig MB, Ma HJ, Vescio RA, Pold M, Schiller G, Belson D, et al. Kaposi’s sarcoma-associated herpesvirus infection of bone marrow dendritic cells from multiple myeloma patients. Science. 1997;276:1851.Google Scholar
  27. 27.
    Dunphy CH, Nies MK, Gabriel DA. Correlation of plasma cell percentages by CD138 immunohistochemistry, cyclin D1 status, and CD56 expression with clinical parameters and overall survival in plasma cell myeloma. Appl Immunohistochem Mol Morphol. 2007;15(3):248–54.PubMedCrossRefGoogle Scholar
  28. 28.
    Chang H, Yeung J, Qi C, Xu W. Aberrant nuclear p53 protein expression detected by immunohistochemistry is associated with hemizygous P53 deletion and poor survival for multiple myeloma. Br J Haematol. 2007;138(3):324–9.PubMedCrossRefGoogle Scholar

Copyright information

© The Japanese Society of Hematology 2008

Authors and Affiliations

  • Mohammad Hadi Sadeghian
    • 1
  • Mehrdad Katebi
    • 2
  • Hossein Ayatollahi
    • 2
  • Mohammad Reza Keramati
    • 2
  1. 1.Hematology and Blood Banking DepartmentFaculty of Medicine, Mashhad University of Medical Science (MUMS)MashhadIran
  2. 2.MUMSMashhadIran

Personalised recommendations