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International Journal of Hematology

, Volume 87, Issue 3, pp 303–309 | Cite as

Molecular genotyping and frequencies of A 1 , A 2 , B, O 1 and O 2 alleles of the ABO blood group system in a Kuwaiti population

  • Mokhtar M. El-Zawahri
  • Yunus A. LuqmaniEmail author
Original Article

Abstract

Expression of the highly polymorphic ABO gene cluster is commonly investigated for blood transfusion and analysis, but little information is available for Middle Eastern populations. This study determined the major ABO allele frequency in a Kuwaiti Arab cohort using a multiplex PCR–RFLP technique; 355 unrelated blood donors of phenotype A1 (46), A2 (31), A1B (6), A2B (4), B (97) and O (171) were genotyped. DNA fragments of 252 (251 for O 1 ) and 843 (842 for A 2 ) bp spanning the two major exons, 6 and 7, of the ABO gene were amplified and digested with HpaII and KpnI. Thirteen different genotypes could be identified when combining the A 1 , A 2 , B, O 1 and O 2 alleles from the digestion patterns: 1 A 1 A 1 (0.28%), 6 A 1 A 2 (1.69%), 38 A 1 O 1 (10.71%), 1 A 1 O 2 (0.28%), 1 A 2 A 2 (0.28%), 30 A 2 O 1 (8.45%), 6 A 1 B (1.69%), 4 A 2 B (1.13%), 12 BB (3.38%), 79 BO 1 (22.25%), 6 BO 2 (1.69%), 167 O 1 O 1 (47.04%) and 4 O 1 O 2 (1.13%). Two of the combinations (A 2 O 2 , O 2 O 2 ) were not found. All genotypes determined were consistent with the serotypes. The frequencies of the five alleles in the Kuwaiti sample population were ABO*A1 = 0.0746, ABO*A2 = 0.0592, ABO*B = 0.1676, ABO*O1 = 0.6831 and ABO*O2 = 0.0155. These results are discussed with reference to gene frequencies reported for other ethnic groups.

Keywords

Molecular genotyping Allele frequencies Suballeles ABO PCR–RFLP Kuwaiti population 

Notes

Acknowledgments

This work was supported by grant SZ 01/00 from Research Administration, Kuwait University, Kuwait. The authors are indebted to Dr Abdul-Aziz Al-Bashir (Director of the Central Blood Bank of Kuwait) for facilitating collection of the blood donor samples and Ms Gilu Abraham for her technical assistance.

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Copyright information

© The Japanese Society of Hematology 2008

Authors and Affiliations

  1. 1.Department of Biological Sciences, Faculty of ScienceKuwait UniversitySafatKuwait
  2. 2.Department of Pharmaceutical Chemistry, Faculty of PharmacyKuwait UniversitySafatKuwait

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