An Immunochromatographic Lateral Flow Strip Test for the Rapid Detection of Danofloxacin in Milk
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An immunochromatographic lateral flow strip test (ILFST) for the rapid detection of danofloxacin (DAN) in milk samples was developed based on colloidal gold-labeled anti-DAN monoclonal antibody 3E9 and a competitive format. Quantitative detections of DAN-spiked samples using the ILFST gave a half inhibitory concentration (IC50) of 0.513 ng/mL and a limit of detection (LOD) of 0.092 ng/mL. Recoveries of DAN from spiked milk samples were from 80.0 to 95.1% within an assay (intra-assay) and from 87.0 to 89.8% between assays (inter-assay). The coefficients of variation (CV) for intra-assay and inter-assay were 2.09–9.93% and 2.42–12.07%, respectively. These data indicated that the ILFST was highly sensitive and reproducible. Detections of authentic milk samples using ILFST and high-performance liquid chromatography (HPLC) showed no significant differences between the two methods. Hence, the ILFST can be widely used for both qualitative and quantitative detection of DAN residues in milk samples.
KeywordsDanofloxacin Monoclonal antibody Colloidal gold Immunochromatographic lateral flow strip test Rapid and quantitative test
This study was supported by the National Science & Technology Pillar Program of the 12th Five-Year Plan (Grant No. 2014BAD13B05), the Natural Science Foundation of Henan Province (Grant No. 182300410093), and Henan Province Science and Technology Agency (Grant No. 182102310683).
Compliance with Ethical Standards
Conflict of Interest
Xingdong Yang declares that he has no conflict of interest. Yinbiao Wang declares that he has no conflict of interest. Jifei Yang declares that he has no conflict of interest. Zhongke Sun Wang declares that he has no conflict of interest. Zonghao Yue declares that he has no conflict of interest. Lili Li declares that she has no conflict of interest. Le He declares that he has no conflict of interest. Xiaofei Hu declares that he has no conflict of interest.
This article does not contain any studies with human participants performed by any of the authors. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
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