Food Analytical Methods

, Volume 13, Issue 1, pp 119–130 | Cite as

Simultaneous Electroanalytical Determination of Thiram and Carbendazim in Samples of Fresh Fruit Juices in the Presence of Surfactants

  • Elizabete M. Maximiano
  • Claudia A. L. Cardoso
  • Gilberto J. ArrudaEmail author


This study describes a new simple electrochemical method, with high sensitivity and selectivity to simultaneously quantify both thiram (THI) and carbendazim (MBC) pesticides in samples of apple, banana, peach, and strawberry juices, using carbon paste electrode modified with thermally activated zeolite in the presence of surfactants. Differential pulse voltammograms obtained for THI and MBC indicated irreversible behavior at approximately 700 mV and 800 mV (vs. Ag/AgCl (3 mol L−1 KCl)), respectively, when the anionic surfactant was present in the electrochemical cell. Values of peak oxidation currents exhibited linear correlations in the concentration ranges 3.44–1,000 and 1.72–350 ng mL−1 for THI and MBC, respectively. Limits of detection and quantification were estimated by obtaining the values 1.01 and 0.51 and 3.38 and 1.67 ng mL−1 for THI and MBC, respectively. After the sample contamination, recoveries were obtained between 98.6% and 101.8%. Values of relative standard deviation obtained in the stability, repeatability, precision, and selectivity studies were lower than 2% for both pesticides. Recoveries obtained with the proposed methodology showed high conformity with the results obtained using high-performance liquid chromatography (HPLC).


Fungicides Zeolite Carbon paste electrode Surfactants Foods Differential pulse voltammetry 



The authors would like to thank Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the scholarship granted to Elizabete Maria Maximiano (Programa Demanda Social - CAPES) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (CALC, grant number 310801/2015-0), FUNDECT, and FINEP.

Compliance with Ethical Standards

Conflict of Interest

Elizabete M. Maximiano declares that she has no conflict of interest. Claudia A. L. Cardoso declares that she has no conflict of interest. Gilberto J. Arruda declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human or animal subjects.

Informed Consent

Not applicable.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Elizabete M. Maximiano
    • 1
  • Claudia A. L. Cardoso
    • 1
  • Gilberto J. Arruda
    • 1
    Email author
  1. 1.Graduate Program in Natural ResourcesUniversidade Estadual de Mato Grosso do SulDouradosBrazil

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