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Solid-Phase Extraction Combined with Dispersive Liquid-Liquid Microextraction Based on Solidification of Floating Organic Droplet for Simultaneous Determination of Organochlorine Pesticides and Polychlorinated Biphenyls in Fish

  • Lijun Xu
  • Xiaohuan Miao
  • Zhaoguang Yang
  • Haipu LiEmail author
  • Bo QiuEmail author
Article
  • 5 Downloads

Abstract

Solid-phase extraction (SPE) was combined with dispersive liquid-liquid microextraction based on solidification of floating organic droplet (DLLME-SFO) as a pretreatment technique for the simultaneous analysis of 11 organochlorine pesticides (OCPs) and 5 polychlorinated biphenyls (PCBs) in fish tissue. Gas chromatography with electron capture detector was used for the identification and quantification of these compounds. In the newly developed method, the acetonitrile extractant of the fish sample was cleaned up by commercialized SPE cartridges packed with acid alumina, and then mixed with water, sodium-chloride, and n-pentadecane for the purpose of target analyte enrichment via DLLME-SFO process. Effect of the parameters including SPE packing material, amount and chemical nature of extraction solvent, water volume, and salt concentration on the signal responses were investigated, and the recoveries of this method ranged between 70.1% and 112.3% at three spiked levels under the optimized conditions. The calibration curves were found to be linear in the range of 1.56–312.5 μg L−1, and the limits of detection ranged from 0.372 to 1.12 μg kg−1. Comparing the other reported pretreatment method, the presented one was sensitive, time-saving, and environmentally friendly.

Keywords

Dispersive liquid-liquid microextraction based on solidification of floating organic droplet Fish Gas chromatography with electron capture detection Organochlorine pesticides Polychlorinated biphenyls Solid-phase extraction 

Notes

Acknowledgments

This work was supported by the National Special Fund for Agro-scientific Research in the Public Interest (No. 201503108) and Hydraulic Science & Technology Project of Hunan Province(No. 2017-230-13).

Compliance with Ethical Standards

Conflict of Interest

Lijun Xu declares that he has no conflict of interest. Xiaohuan Miao declares that he has no conflict of interest. Zhaoguang Yang declares that he has no conflict of interest. Haipu Li declares that he has no conflict of interest. Bo Qiu declares that he has no conflict of interest.

Ethical Approval

This work does not involve any experiments conducted on human or animal participants.

Informed Consent

Not applicable.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Center for Environment and Water Resources/College of Chemistry and Chemical EngineeringCentral South UniversityChangshaPeople’s Republic of China
  2. 2.Key Laboratory of Hunan Province for Water Environment and Agriculture Product SafetyChangshaPeople’s Republic of China
  3. 3.Changsha Animal Disease Prevention and Control CenterChangshaPeople’s Republic of China

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