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Urazole-Au Nanocluster as a Novel Fluorescence Probe for Curcumin Determination and Mitochondria Imaging

  • Rui Yang
  • Wei-Yu Mu
  • Qiu-Yun ChenEmail author
Article
  • 9 Downloads

Abstract

Curcumin (CUR), a natural plant product, is widely used in food and medicine. The determination of CUR in medicine and food will be important to control the quality of CUR containing products. Herein, a new kind of fluorescence urazole-Au clusters (Urazole-AuNCs) has been synthesized successfully via one-step method using urazole and polyvinyl pyrrolidone (PVP) as templates. The fluorescence emission intensity of Urazole-AuNCs at 473 nm is sensitive to the concentration of CUR. Therefore, a fluorescence method for the quantitative detection of CUR was built using Urazole-AuNCs as the fluorescence sensor with detection limit of 72 nM. This method was successfully used to quantitatively monitor CUR in curry powder; Urazole-AuNC-based fluorescence method can be a good technique for quantitative detection of CUR and differentiation of CUR from others. Furthermore, the novel low-toxic urazole-Au clusters could be used for mitochondrial targeted imaging of cancer cells.

Keywords

Urazole AuNCs Curcumin Detection Fluorescence Technique 

Notes

Funding Information

This work was financially supported by the National Natural Science Foundation of China (21571085).

Compliance with Ethical Standards

Conflict of Interest

Rui Yang declares that he has no conflict of interest. Weiyu Mu declares that she has no conflict of interest. Qiuyun Chen declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

Supplementary material

12161_2019_1519_MOESM1_ESM.doc (656 kb)
ESM 1 (DOC 656 kb)

References

  1. Bian W, Zhang H, Yu Q, Shi MJ, Shuang SM, Cai ZW, Choi MMF (2016) Detection of Ag+ using graphite carbon nitride nanosheets based on fluorescence quenching. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 169:122–127.  https://doi.org/10.1016/j.saa.2016.06.024 CrossRefGoogle Scholar
  2. Bian W, Wang X, Wang YK, Yang HF, Huang JL, Cai ZW, Choi MMF (2018) Boron and nitrogen co-doped carbon dots as a sensitive fluorescent probe for the detection of curcumin. Luminescence 33(1):174–180.  https://doi.org/10.1002/bio.3390 CrossRefGoogle Scholar
  3. Brouet I, Ohshima H (1995) Curcumin, an anti-tumour promoter and anti-inflammatory agent, inhibits induction of nitric oxide synthase in activated macrophages. Biochem Biophys Res Commun. 206(2):533–540.  https://doi.org/10.1006/bbrc.1995.1076 CrossRefGoogle Scholar
  4. Cho KH, Choo J, Joo SW (2005) Tautomerism of thymine on gold and silver nanoparticle surfaces: surface-enhanced Raman scattering and density functional theory calculation study. J. Mol. Structure 738(1–3):9–14.  https://doi.org/10.1016/j.molstruc.2004.11.001 CrossRefGoogle Scholar
  5. Das L, Vinayak M (2014) Long term effect of curcumin in regulation of glycolytic pathway and angiogenesis via modulation of stress activated genes in prevention of cancer. Plos One 9(6):99583–99595.  https://doi.org/10.1371/journal.pone.0099583 CrossRefGoogle Scholar
  6. Deng WQ, Dai R, Hu PY, Li QQ, Xiong XL, Huang K, Huo F (2018) A traffic light-type sensitive visual detection of mercury by golden nanoclusters mixed with fluorescein. Microchemical Journal 141:163–169.  https://doi.org/10.1016/j.microc.2018.05.026 CrossRefGoogle Scholar
  7. Garcea G, Jones DJL, Singh R, Dennison AR, Farmer PB, Sharma RA, Steward WP, Gescher AJ, Berry DP (2004) Detection of curcumin and its metabolites in hepatic tissue and portal blood of patients following oral administration. Brit. J. Cancer 90(5):1011–1015.  https://doi.org/10.1038/sj.bjc.6601623 CrossRefGoogle Scholar
  8. Goel A, Kunnumakkara AB, Aggarwal BB (2008) Curcumin as “Curecumin”: from kitchen to clinic. Biochem. Pharmacol. 75(4):787–809.  https://doi.org/10.1016/j.bcp.2007.08.016 CrossRefGoogle Scholar
  9. Hamzehzadeh L, Atkin SL, Majeed M, Butler AE, Sahebkar A (2018) The versatile role of curcumin in cancer prevention and treatment: a focus on PI3K/AKT pathway. J. Cell. Physiol. 233(10):6530–6537.  https://doi.org/10.1002/jcp.26620 CrossRefGoogle Scholar
  10. Han Z, Zhang H, He L, Pan S, Liu H, Hu XL (2019) One-pot hydrothermal synthesis of nitrogen and sulfur co-doped carbon dots and their application for sensitive detection of curcumin and temperature. Microchemical Journal 146:300–308.  https://doi.org/10.1016/j.microc.2019.01.024 CrossRefGoogle Scholar
  11. Hu Q, Gao L, Rao SQ, Yang ZQ, Li T, Gong XJ (2019) Nitrogen and chlorine dual-doped carbon nanodots for determination of curcumin in food matrix via inner filter effect. Food Chemistry 280:195–202.  https://doi.org/10.1016/j.foodchem.2018.12.050 CrossRefGoogle Scholar
  12. Jadhav BK, Mahadik KR, Paradkar AR (2007) Development and validation of improved reversed phase-HPLC method for simultaneous determination of curcumin, demethoxycurcumin and bis-demethoxycurcumin. Chromatographia 65(7–8):483–488.  https://doi.org/10.1365/s10337-006-0164-8nani CrossRefGoogle Scholar
  13. Kawasaki H, Yamamoto H, Fujimori H, Arakawa R, Iwasaki Y, Inada M (2010) Stability of the DMF-protected Au nanoclusters: photochemical, dispersion, and thermal properties. Langmuir 26(8):5926–5933.  https://doi.org/10.1021/la9038842 CrossRefGoogle Scholar
  14. Ler SE, Campbell BC, Molyneux RJ, Hasegawa S, Lee HS (2001) Inhibitory effects of naturally occurring compounds on aflatoxin B(1) biotransformation. J. Agric. Food Chem. 49(11):5171–5177.  https://doi.org/10.1021/jf010454v CrossRefGoogle Scholar
  15. Li KJ, Li YF, Yang LX, Wang L, Ye BX (2014) The electrochemical characterization of curcumin and its selective detection in curcuma using a graphene-modified electrode. Anal. Methods. 6(19):7801–7808.  https://doi.org/10.1039/C4AY01492H CrossRefGoogle Scholar
  16. Lin H, Li L, Lei C, Xu X, Nie Z, Guo M, Huang Y, Yao S (2013) Immune-independent and label-free fluorescent assay for cystatin c detection based on protein-stabilized Au nanoclusters. Biosensors and Bioelectronics 41:256–261.  https://doi.org/10.1016/j.bios.2012.08.030 CrossRefGoogle Scholar
  17. Liu Y, Han S (2017) Chemiluminescence of nitrogen-doped carbon quantum dots for the determination of thiourea and tannic acid. Food Anal Methods 10:3398–3406.  https://doi.org/10.1007/s12161-017-0911-5 CrossRefGoogle Scholar
  18. Liu YJ, Cai YX, Jiang XY, Wu JP, Le XY (2016) Molecular interactions, characterization and antimicrobial activity of curcumin-chitosan blend films. Food Hydrocolloids 52:564–572.  https://doi.org/10.1016/j.foodhyd.2015.08.005
  19. Mu WY, Yang R, Robertson R, Chen QY (2018) A near-infrared BSA coated DNA-AgNCs for cellular imaging. Colloids Surf. B: Biointerf. 162:427–431.  https://doi.org/10.1016/j.colsurfb.2017.12.023 CrossRefGoogle Scholar
  20. Roy SD, Ghosh M, Chowdhury J (2015) How hottest geometries and adsorptive parameters influence the SER(R) S spectra of methylene blue molecules adsorbed on nanocolloidal gold particles of varied sizes. Spectrochim. Acta A: Mol. Biomol. Spectros. 151:796–806.  https://doi.org/10.1016/j.saa.2015.06.125 CrossRefGoogle Scholar
  21. Shang L, Yang L, Stockmar F, Popescu R, Trouillet V, Bruns M, Gerthsen D, Nienhaus GU (2012) Microwave-assisted rapid synthesis of luminescent gold nanoclusters for sensing Hg2+ in living cells using fluorescence imaging. Nanoscale 4:4155–4160.  https://doi.org/10.1039/c2nr30219e CrossRefGoogle Scholar
  22. Sun XH, Gao CL, Cao WD, Yang XR, Wang EK (2002) Capillary electrophoresis with amperometric detection of curcumin in Chinese herbal medicine pretreated by solid-phase extraction. J. Chromatogr. A 962(1–2):117–125.  https://doi.org/10.1016/S0021-9673(02)00509-5 CrossRefGoogle Scholar
  23. Syed HK, Liew KB, Loh GO, Peh KK (2015) Stability indicating HPLC-UV method for detection of curcumin in Curcuma longa extract and emulsion formulation. Food Chem. 170:321–326.  https://doi.org/10.1016/j.foodchem.2014.08.066 CrossRefGoogle Scholar
  24. Tao Y, Lin Y, Ren J, Qu X (2013) A dual fluorometric and colorimetric sensor for dopamine based on BSA-stabilized Au nanoclusters. Biosens. Bioelectron. 42:41–46.  https://doi.org/10.1016/j.bios.2012.10.014 CrossRefGoogle Scholar
  25. Wang J, Du XX, Jiang H, Xie J (2009) Curcumin attenuates 6-hydroxydopamine-induced cytotoxicity by anti-oxidation and nuclear factor-kappa B modulation in MES23.5 cells. Biochem. Pharmacol. 78(2):178–183.  https://doi.org/10.1016/j.bcp.2009.03.031 CrossRefGoogle Scholar
  26. Wang CX, Lin HH, Xu ZZ, Huang YJ, Humphrey MG, Zhang C (2016) Tunable carbon-dot-based dual-emission fluorescent nanohybrids for ratiometric optical thermometry in living cells. ACS Appl. Mater. Interfaces. 8(10):6621–6628.  https://doi.org/10.1021/acsami.5b11317 CrossRefGoogle Scholar
  27. Wang J, Wang H, Yang S, Tian H, Liu Y, Sun B (2018) A novel fluorescent probe for detecting hydrogen sulfide in wine. Food Anal Methods 11:1398–1404.  https://doi.org/10.1007/s12161-017-1124-7 CrossRefGoogle Scholar
  28. Xu YW, Zhang W, Shi JY, Zou XB, Li YX (2017) Electrodeposition of gold nanoparticles and reduced graphene oxide on an electrode for fast and sensitive determination of methylmercury in fish. Food Chem 237:423–430.  https://doi.org/10.1016/j.foodchem.2017.05.096 CrossRefGoogle Scholar
  29. Yang HF, Li XB, Wang XX, Chen WF, Bian W, Choi MMF (2018) Silver-doped graphite carbon nitride nanosheets as fluorescent probe for the detection of curcumin. Luminescence 33(6):1062–1069.  https://doi.org/10.1002/bio.3509 CrossRefGoogle Scholar
  30. Zhang LB, Wang EK (2014) Metal nanoclusters: New fluorescent probes for sensors and bioimaging. Nano Today 9(1):132–157.  https://doi.org/10.1016/j.nantod.2014.02.010 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Chemistry and Chemical EngineeringJiangsu UniversityZhenjiangPeople’s Republic of China

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