Urazole-Au Nanocluster as a Novel Fluorescence Probe for Curcumin Determination and Mitochondria Imaging
- 9 Downloads
Curcumin (CUR), a natural plant product, is widely used in food and medicine. The determination of CUR in medicine and food will be important to control the quality of CUR containing products. Herein, a new kind of fluorescence urazole-Au clusters (Urazole-AuNCs) has been synthesized successfully via one-step method using urazole and polyvinyl pyrrolidone (PVP) as templates. The fluorescence emission intensity of Urazole-AuNCs at 473 nm is sensitive to the concentration of CUR. Therefore, a fluorescence method for the quantitative detection of CUR was built using Urazole-AuNCs as the fluorescence sensor with detection limit of 72 nM. This method was successfully used to quantitatively monitor CUR in curry powder; Urazole-AuNC-based fluorescence method can be a good technique for quantitative detection of CUR and differentiation of CUR from others. Furthermore, the novel low-toxic urazole-Au clusters could be used for mitochondrial targeted imaging of cancer cells.
KeywordsUrazole AuNCs Curcumin Detection Fluorescence Technique
This work was financially supported by the National Natural Science Foundation of China (21571085).
Compliance with Ethical Standards
Conflict of Interest
Rui Yang declares that he has no conflict of interest. Weiyu Mu declares that she has no conflict of interest. Qiuyun Chen declares that she has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Bian W, Zhang H, Yu Q, Shi MJ, Shuang SM, Cai ZW, Choi MMF (2016) Detection of Ag+ using graphite carbon nitride nanosheets based on fluorescence quenching. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 169:122–127. https://doi.org/10.1016/j.saa.2016.06.024 CrossRefGoogle Scholar
- Garcea G, Jones DJL, Singh R, Dennison AR, Farmer PB, Sharma RA, Steward WP, Gescher AJ, Berry DP (2004) Detection of curcumin and its metabolites in hepatic tissue and portal blood of patients following oral administration. Brit. J. Cancer 90(5):1011–1015. https://doi.org/10.1038/sj.bjc.6601623 CrossRefGoogle Scholar
- Han Z, Zhang H, He L, Pan S, Liu H, Hu XL (2019) One-pot hydrothermal synthesis of nitrogen and sulfur co-doped carbon dots and their application for sensitive detection of curcumin and temperature. Microchemical Journal 146:300–308. https://doi.org/10.1016/j.microc.2019.01.024 CrossRefGoogle Scholar
- Liu YJ, Cai YX, Jiang XY, Wu JP, Le XY (2016) Molecular interactions, characterization and antimicrobial activity of curcumin-chitosan blend films. Food Hydrocolloids 52:564–572. https://doi.org/10.1016/j.foodhyd.2015.08.005
- Roy SD, Ghosh M, Chowdhury J (2015) How hottest geometries and adsorptive parameters influence the SER(R) S spectra of methylene blue molecules adsorbed on nanocolloidal gold particles of varied sizes. Spectrochim. Acta A: Mol. Biomol. Spectros. 151:796–806. https://doi.org/10.1016/j.saa.2015.06.125 CrossRefGoogle Scholar
- Shang L, Yang L, Stockmar F, Popescu R, Trouillet V, Bruns M, Gerthsen D, Nienhaus GU (2012) Microwave-assisted rapid synthesis of luminescent gold nanoclusters for sensing Hg2+ in living cells using fluorescence imaging. Nanoscale 4:4155–4160. https://doi.org/10.1039/c2nr30219e CrossRefGoogle Scholar