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Food Analytical Methods

, Volume 13, Issue 1, pp 61–68 | Cite as

Phenolic Compounds from Butia odorata (Barb. Rodr.) Noblick Fruit and Its Antioxidant and Antitumor Activities

  • Joana Schuelter BoeingEmail author
  • Érica Oliveira Barizão
  • Eliza Mariane Rotta
  • Hélito Volpato
  • Celso Vataru Nakamura
  • Liane Maldaner
  • Jesuí Vergílio Visentainer
Article

Abstract

This study evaluated the antioxidant activity and total phenolic content (TPC) of Butia odorata fruit extracted with different solvents, as well as the phenolic composition using UHPLC-MS/MS analysis. In addition, the antitumor potential of B. odorata fruit against various cancer cell lines (Caco-2, HeLa, SiHa, C33a) was evaluated for the first time. The TPC and antioxidant activity (DPPH and ORAC assays) varied according to the extraction solvents, with the highest values obtained for the methanol:water (80:20, v/v) extracts. Thirteen phenolic compounds were quantified in the ethanol:water (80:20, v/v) extracts of B. odorata fruit by UHPLC-MS/MS, with the main compounds identified as (+)-catechin (259 ± 18 mg kg−1), (−)-epicatechin (211 ± 12 mg kg−1) and rutin (161 ± 2 mg kg−1). Sinapic and ellagic acids, trans-resveratrol, naringenin, and apigenin were reported in B. odarata fruit for the first time. Furthermore, the B. odorata fruit showed antitumor activity against cervical cancer cell lines (SiHa and C33a), providing preliminary evidence for further assessment of its anticancer potential.

Keywords

Butia odorata fruit Extraction solvent UHPLC-MS/MS Cancer cells Arecaceae Phenolic composition 

Notes

Acknowledgments

The authors thank MSc. Kelen Pureza Soares and the Herbarium of the State University of Maringá for the identification of the B. odorata plant.

Funding Information

This work received financial support from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

Supplementary material

12161_2019_1515_MOESM1_ESM.docx (67 kb)
ESM 1 (DOCX 67 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Joana Schuelter Boeing
    • 1
    • 2
    Email author
  • Érica Oliveira Barizão
    • 1
  • Eliza Mariane Rotta
    • 1
  • Hélito Volpato
    • 3
  • Celso Vataru Nakamura
    • 3
    • 4
  • Liane Maldaner
    • 1
  • Jesuí Vergílio Visentainer
    • 1
  1. 1.Department of ChemistryState University of MaringáParanáBrazil
  2. 2.Faculty of Exact Sciences and Technology (FACET)Federal University of Grande DouradosDouradosBrazil
  3. 3.Post-Graduate Program in Biological Sciences, Cellular and Molecular Biological Concentration AreaState University of MaringáParanáBrazil
  4. 4.Post-Graduate Program in Pharmaceutical SciencesState University of MaringáParanáBrazil

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