Food Analytical Methods

, Volume 13, Issue 1, pp 35–43 | Cite as

Evaluation of Lipoxygenase Activity in Common Beans by UV and NMR Spectroscopy: Proposal for a Complementary Technique for Enzymatic Studies

  • Andressa Kuhnen Silva
  • Gerlon de Almeida Ribeiro Oliveira
  • Priscila Zaczuk Bassinello
  • Anna Cristina Lanna
  • Etelvino Henrique Novotny
  • Luciano Morais LiãoEmail author


Factors that reduce the quality of common bean during storage, including oxidative rancidity caused by lipoxygenase, have been extensively studied. In this context, this work evaluates lipoxygenase activity in five Carioca bean cultivars at different temperatures and storage times using ultraviolet (UV) and nuclear magnetic resonance (NMR) spectroscopies. UV absorption analyses demonstrated that the storage temperature of the grains influences the kinetic reaction rates during the enzymatic activity of lipoxygenase. Pinto beans and CNFC 10467 presented lower initial activity than the three BRS varieties but were more sensitive to high temperatures. BRS Estilo presented an anomalous behavior, with a strong reduction in activity at 15 and 21 °C until 72 days of storage. BRSMG Madrepérola and BRS Pontal presented stable enzymatic rates until 108 days of storage. A 26% reduction in fatty acid content, as a result of lipoxygenase action during storage, was observed both in solution-state and high resolution magic angle spinning (HR-MAS) NMR studies. These results highlight the importance of NMR in enzymatic kinetic reactions studies, complementing the results obtained by UV.


Phaseolus vulgaris Oxidative enzyme Storage conditions Solution-state NMR 1H HR-MAS NMR 



The authors are grateful to the Federal University of Goiás (UFG) for infrastructure support, as well as Financiadora de Estudos e Projetos (FINEP), Fundação de Amparo à Pesquisa do Estado de Goiás (FAPEG), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for continued financial support and scholarships.


Embrapa for funding the project DarkBean. The Financier of Studies and Projects (FINEP) approved the purchase of NMR; the Foundation for Research Support of the State of Goiás (FAPEG) and the National Council for Scientific and Technological Development (CNPq) supported other projects, which indirectly bring resources to the laboratory. Thus, they are in Acknowledgments section.

Compliance with Ethical Standards

Conflict of Interest

Andressa Kuhnen Silva declares that he has no conflict of interest. Gerlon de Almeida Ribeiro Oliveira declares that he has no conflict of interest. Priscila Zaczuk Bassinello declares that she has no conflict of interest. Anna Cristina Lanna declares that she has no conflict of interest. Etelvino Henrique Novotny declares that he has no conflict of interest. Luciano Morais Lião declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Andressa Kuhnen Silva
    • 1
  • Gerlon de Almeida Ribeiro Oliveira
    • 1
  • Priscila Zaczuk Bassinello
    • 2
  • Anna Cristina Lanna
    • 2
  • Etelvino Henrique Novotny
    • 3
  • Luciano Morais Lião
    • 1
    Email author
  1. 1.Instituto de Química, Laboratorio de RMNUniversidade Federal de GoiásGoiâniaBrazil
  2. 2.Embrapa Arroz e FeijãoSanto Antônio de GoiásBrazil
  3. 3.Embrapa SolosRio de JaneiroBrazil

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