Lab-On-A-Chip Extraction of Phenolic Compounds from Extra Virgin Olive Oil

  • Kenia Chávez Ramos
  • Luis Fernando Olguín Contreras
  • María del Pilar Cañizares MacíasEmail author


A novel liquid–liquid extraction method of phenolic compounds from extra virgin olive using microfluidic technique was developed. The microdevice of polydimethylsiloxane (PDMS) was designed in two parts; the first one to carry out the extraction of polyphenols using only an alkaline aqueous solution and the second one to develop the reaction product using Folin–Ciocalteu reagent, which was measured at 730 nm at the microchip exit. Hydrodynamic and chemical parameters, such as flowrates of extraction and reaction, length of microchannels, extraction pH, extraction buffer concentration, and concentration of Folin–Ciocalteu reagent, were evaluated. Although all parameters were important, the results showed that pH, carbonate buffer concentration, and the Folin–Ciocalteu reagent concentration were significant factors and that the increase in the length of the extraction coil enhanced the extraction percentage. The results showed higher extraction efficiency by the microfluidic method, between 46 and 67%, than for the other two batch extraction methods.


Polyphenols Lab-on-a-chip Folin–Ciocalteu assay Liquid–liquid microfluidic extraction Extra virgin olive oil 



The authors are truly grateful to Dirección General de Asuntos del Personal Académico (DGAPA) (Project Number IN218415) and to Facultad de Química of Universidad Nacional Autónoma de México (Programa de Apoyo a la Investigación y el Posgrado) (PAIP 5000-9029 and 5000-9023), for their contribution and financial support.

The authors are also grateful to Minerva Monrroy Barreto, PhD, from Unidad de Apoyo a la Investigación y a la Industria (USAII), Facultad de Química, for carrying out the HPLC analyses.

Compliance with Ethical Standards

Conflict of Interest

Kenia Chávez-Ramos declares that she has no conflict of interest. Luis Fernando Olguín-Contreras declares that he has no conflict of interest. María del Pilar Cañizares-Macías declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

Supplementary material

12161_2019_1492_MOESM1_ESM.docx (791 kb)
ESM 1 (DOCX 791 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Kenia Chávez Ramos
    • 1
  • Luis Fernando Olguín Contreras
    • 2
  • María del Pilar Cañizares Macías
    • 1
    Email author
  1. 1.Departamento de Química Analítica, Facultad de QuímicaUniversidad Nacional Autónoma de MéxicoMéxico D.F.Mexico
  2. 2.Laboratorio de Biofisicoquímica, Facultad de QuímicaUniversidad Nacional Autónoma de MéxicoMéxico D.F.Mexico

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