AuNP Tetramer-Based Aptasensor for SERS Sensing of Oxytetracycline

  • Zhengzong WuEmail author


A novel aptasensor was described and employed for ultrasensitive determination of the antibacterial agent oxytetracycline (OTC). This method was based on gold nanoparticle (AuNP) tetramers and surface-enhanced Raman spectroscopy (SERS). Under optimal conditions, in the range of 0.01–250 ng/mL, the concentration of OTC exhibited good linear relationship (R2 = 0.9948) with changed SERS signal intensity. In addition, the applicability of the novel aptasensor was validated by detecting different concentrations of OTC spiked in milk samples. With its satisfactory performances, this aptasensor is perceived to be a promising candidate for determining OTC in food products. This assay could also be extended for the analysis of other targets (pathogens or harmful substances) in food products by using corresponding DNA sequences.


Oxytetracycline Surface-enhanced Raman spectroscopy Rapid detection Antibiotic residues Gold nanoparticles 


Funding Information

This research was supported by Natural Science Foundation of Shandong Province and Special Funds for Taishan Scholars Project.

Compliance with Ethical Standards

Conflict of Interest

Zhengzong Wu declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human or animal subjects.

Informed Consent

Informed consent was obtained from all individual participants included in the study.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Biobased Material and Green PapermakingQilu University of Technology, Shandong Academy of SciencesJinanChina
  2. 2.School of Food Science and EngineeringQilu University of Technology, Shandong Academy of SciencesJinanChina

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