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Reliability Evaluation of MALDI-TOF MS Associated with SARAMIS Software in Rapid Identification of Thermophilic Campylobacter Isolated from Food

  • Graziella Ziino
  • Stefania Maria Marotta
  • Filippo GiarratanaEmail author
  • Alessandro Giuffrida
  • Felice Panebianco
Article

Abstract

Thermophilic Campylobacter species represent the most frequent cause of food-borne disease worldwide. Biochemical and serological tests, according to ISO 10272:2017, are routinely used in microbiology laboratories for their detection/identification. However, these procedures require a lot of time and can incur in diagnostic mistakes. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is considered a promising identification method for bacteria and fungi from clinical isolates. The aim of this study was to evaluate the reliability of MALDI-TOF MS associated with SARAMIS software, in rapid identification of food-related Campylobacter spp., considering that this method was tested only on human and animal clinical strains. A total of 276 putative thermophilic Campylobacter strains, isolated from different foodstuffs according to ISO 10272-1:2006, were identified comparing the results of MALDI-TOF MS/SARAMIS with Multiplex PCR assay. MALDI-TOF MS/SARAMIS correctly identified 96% (n = 267) of strains at the species level, while only 2.5% (n = 7) of strains were not identified. Misidentification occurred in 0.7% (n = 2) of samples. The performance of this method demonstrated an overall sensitivity (Se) of 97.45% and specificity (Sp) of 100%. Thus, this method can be used for the rapid identification of Campylobacter strains isolated from food samples according to ISO 10272:2017.

Keywords

MALDI-TOF MS SARAMIS TM Campylobacter Rapid identification Food strains 

Notes

Compliance with Ethical Standards

Conflict of Interest

Graziella Ziino declares that she has no conflict of interest. Stefania Maria Marotta declares that she has no conflict of interest. Filippo Giarratana declares that he has no conflict of interest. Alessandro Giuffrida declares that he has no conflict of interest. Felice Panebianco declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Veterinary SciencesUniversity of MessinaMessinaItaly
  2. 2.Department of Agraria“Mediterranea” University of Reggio CalabriaReggio CalabriaItaly

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