Simultaneous Detection of Listeria monocytogenes and Salmonella typhimurium by a SERS-Based Lateral Flow Immunochromatographic Assay
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Foodborne diseases arise from Listeria monocytogenes (L. monocytogenes) and Salmonella typhimurium (S. typhimurium) are global public health problems, influencing both developing and developed countries. Therefore, their rapid and accurate detection is of much concern in food safety and point-of-care diagnostics. In this study, a surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) for simultaneous detection of them was developed. Under optimal conditions, the proposed assay showed good linear responses in the ranges of 102–107 cfu/mL and 102–107 cfu/mL with limits of detection of 75 cfu/mL and 75 cfu/mL, respectively, for L. monocytogenes and S. typhimurium, respectively. The practicality of the LFA in real food sample (milk) was also validated. Overall, the proposed assay is promising and has a good chance to be employed for multiple pathogen detection in food products.
KeywordsFood analysis Surface-enhanced Raman scattering Multiplex detection Pathogen Lateral flow
This research was supported by the Postgraduate research and Practice Innovation Program of Jiangsu Province (No. KYCX18_751), Nature Science Foundation of Jiangsu Province (No. BK20160168), Nature Science Foundation of China (Nos. 31601413 and 31501418) and Special Funds for Taishan Scholars Project.
Compliance with Ethical Standards
Conflict of Interest
Zhengzong Wu declares that he has no conflict of interest.
This article does not contain any studies with human or animal subjects.
Informed consent was obtained from all individual participants included in the study.
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