Monolithic Material Prepared with Nanodiamond as Monomer for the Enrichment of β-Sitosterol in Edible Oil
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In this paper, a hybrid monolithic column with modified nanodiamond and 1-dodecene as co-monomers was prepared and applied to the online enrichment and purification of β-sitosterol in edible oil. The obtained monolithic column was characterized by scanning electron microscopy (SEM) and nitrogen adsorption–desorption isotherm measurement, which indicated that the monolith possessed characteristics of porous structure and high permeability. Under the optimum conditions for extraction and determination, the linear regression coefficient for β-sitosterol was 0.998; the limit of detection (LOD) and the limit of quantitation (LOQ) were 3.0 μg mL−1 and 10.0 μg mL−1, respectively; the relative standard deviation (RSD) of intra-day and inter-day assays for four edible oils were 2.33–6.58% and 3.24–6.94%, respectively. The recovery was in the range of 89.6–105.5%. The results showed that the hybrid monolithic column with high selectivity and good permeability were successfully used as online solid-phase extraction (SPE) column for determination of β-sitosterol in edible oil.
KeywordsNanodiamond Hybrid column Enrichment β-Sitosterol
The work was supported by the National Natural Science Foundation of China (nos. 21575033, 21505030).
Compliance of Ethical Standards
Conflict of Interest
Beijiao Cui declares that he has no conflict of interest. Huan Yu declares that he has no conflict of interest. Xiaoya Pang declares that he has no conflict of interest. Hongyuan Yan declares that he has no conflict of interest. Ligai Bai declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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