Food Analytical Methods

, Volume 12, Issue 3, pp 697–704 | Cite as

Monolithic Material Prepared with Nanodiamond as Monomer for the Enrichment of β-Sitosterol in Edible Oil

  • Beijiao Cui
  • Haiyan LiuEmail author
  • Huan Yu
  • Xiaoya Pang
  • Hongyuan Yan
  • Ligai BaiEmail author


In this paper, a hybrid monolithic column with modified nanodiamond and 1-dodecene as co-monomers was prepared and applied to the online enrichment and purification of β-sitosterol in edible oil. The obtained monolithic column was characterized by scanning electron microscopy (SEM) and nitrogen adsorption–desorption isotherm measurement, which indicated that the monolith possessed characteristics of porous structure and high permeability. Under the optimum conditions for extraction and determination, the linear regression coefficient for β-sitosterol was 0.998; the limit of detection (LOD) and the limit of quantitation (LOQ) were 3.0 μg mL−1 and 10.0 μg mL−1, respectively; the relative standard deviation (RSD) of intra-day and inter-day assays for four edible oils were 2.33–6.58% and 3.24–6.94%, respectively. The recovery was in the range of 89.6–105.5%. The results showed that the hybrid monolithic column with high selectivity and good permeability were successfully used as online solid-phase extraction (SPE) column for determination of β-sitosterol in edible oil.


Nanodiamond Hybrid column Enrichment β-Sitosterol 


Funding information

The work was supported by the National Natural Science Foundation of China (nos. 21575033, 21505030).

Compliance of Ethical Standards

Conflict of Interest

Beijiao Cui declares that he has no conflict of interest. Huan Yu declares that he has no conflict of interest. Xiaoya Pang declares that he has no conflict of interest. Hongyuan Yan declares that he has no conflict of interest. Ligai Bai declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical SciencesHebei UniversityBaodingChina
  2. 2.Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of EducationBaodingChina
  3. 3.College of Public HealthHebei UniversityBaodingChina

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