Building a Fluorescent Aptasensor Based on Exonuclease-Assisted Target Recycling Strategy for One-Step Detection of T-2 Toxin
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In this work, a rapid and accurate assay was successfully developed for T-2 toxin detection based on exonuclease-catalyzed target recycling strategy. Upconversion nanoparticles (UCNPs) were conjugated with T-2 aptamer and used as signal probes, while magnetic nanoparticles (MNPs) were conjugated with the complementary DNA of T-2 aptamer (cDNA) and used as capture probes. The results reveled that good linear correlation (R2 = 0.9988) was achieved for T-2 toxin detection over the concentration range of 0.1–100 ng/mL with a detection limit as low as 0.035 ng/mL (S/N = 3). In addition, the reliability of the proposed method was also applied to the determination of T-2 toxin contents in real food samples and the average recoveries ranged from 95.97 to 104.00%. The sensing platform developed in our study demonstrated great potential for simple and sensitive detection of T-2 toxin contents in food samples.
KeywordsFluorescence Aptasensor T-2 toxin Target recycling strategy Food safety
This research was supported by the Nature Science Foundation of Jiangsu Province (No. BK20160168), Nature Science Foundation of China (Nos. 31601413 and 31501418), and Special Funds for Taishan Scholars Project.
Compliance with Ethical Standards
Conflict of Interest
Deyun He declares that she has no conflict of interest. Zhengzong Wu declares that he has no conflict of interest. Bo Cui declares that he has no conflict of interest. Enbo Xu declares that he has no conflict of interest. Zhengyu Jin declares that he has no conflict of interest.
This article does not contain any studies with human or animal subjects.
Informed consent was obtained from all individual participants included in the study.
- Deng Q, Qiu M, Wang Y, Lv P, Wu C, Sun L, Ye R, Xu D, Liu Y, Gooneratne R (2017) A sensitive and validated immunomagnetic-bead based enzyme-linked immunosorbent assay for analyzing total T-2 (free and modified) toxins in shrimp tissues. Ecotoxicol Environ Saf 142:441–447. https://doi.org/10.1016/j.ecoenv.2017.04.037 CrossRefGoogle Scholar
- Flores-Flores ME, González-Peñas E (2015) Development and validation of a high performance liquid chromatographic–mass spectrometry method for the simultaneous quantification of 10 trichothecenes in ultra-high temperature processed cow milk. J Chromatogr A 1419:37–44. https://doi.org/10.1016/j.chroma.2015.09.069 CrossRefGoogle Scholar
- Foubert A, Beloglazova NV, Gordienko A, Tessier MD, Drijvers E, Hens Z, De Saeger S (2016) Development of a rainbow lateral flow immunoassay for the simultaneous detection of four mycotoxins. J Agric Food ChemGoogle Scholar
- Kong W, Zhang X, Shen H, Ou-Yang Z, Yang M (2012) Validation of a gas chromatography-electron capture detection of T-2 and HT-2 toxins in Chinese herbal medicines and related products after immunoaffinity column clean-up and pre-column derivatization. Food Chem 132:574–581. https://doi.org/10.1016/j.foodchem.2011.10.073 CrossRefGoogle Scholar
- Porricelli ACR, Lippolis V, Valenzano S, Cortese M, Suman M, Zanardi S, Pascale M (2016) Optimization and validation of a fluorescence polarization immunoassay for rapid detection of T-2 and HT-2 toxins in cereals and cereal-based products. Food Anal Methods 9:3310–3318. https://doi.org/10.1007/s12161-016-0527-1 CrossRefGoogle Scholar
- Stanford GK, Hood RD, Hayes AW (1975) Effect of prenatal administration of T-2 toxin to mice. Res Commun Chem Pathol Pharmacol 10:743–746Google Scholar
- Su Q, Feng W, Yang D, Li F (2016) Resonance energy transfer in upconversion nanoplatforms for selective biodetection. Acc Chem ResGoogle Scholar
- Vilela P, El-Sagheer A, Millar TM, Brown T, Muskens OL, Kanaras AG (2016) Graphene oxide-upconversion nanoparticle based optical sensors for targeted detection of mRNA biomarkers present in Alzheimer’s disease and prostate cancer. ACS SensorsGoogle Scholar