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Food Analytical Methods

, Volume 12, Issue 2, pp 625–632 | Cite as

Building a Fluorescent Aptasensor Based on Exonuclease-Assisted Target Recycling Strategy for One-Step Detection of T-2 Toxin

  • Deyun He
  • Zhengzong WuEmail author
  • Bo CuiEmail author
  • Enbo Xu
  • Zhengyu Jin
Article
  • 97 Downloads

Abstract

In this work, a rapid and accurate assay was successfully developed for T-2 toxin detection based on exonuclease-catalyzed target recycling strategy. Upconversion nanoparticles (UCNPs) were conjugated with T-2 aptamer and used as signal probes, while magnetic nanoparticles (MNPs) were conjugated with the complementary DNA of T-2 aptamer (cDNA) and used as capture probes. The results reveled that good linear correlation (R2 = 0.9988) was achieved for T-2 toxin detection over the concentration range of 0.1–100 ng/mL with a detection limit as low as 0.035 ng/mL (S/N = 3). In addition, the reliability of the proposed method was also applied to the determination of T-2 toxin contents in real food samples and the average recoveries ranged from 95.97 to 104.00%. The sensing platform developed in our study demonstrated great potential for simple and sensitive detection of T-2 toxin contents in food samples.

Keywords

Fluorescence Aptasensor T-2 toxin Target recycling strategy Food safety 

Notes

Funding information

This research was supported by the Nature Science Foundation of Jiangsu Province (No. BK20160168), Nature Science Foundation of China (Nos. 31601413 and 31501418), and Special Funds for Taishan Scholars Project.

Compliance with Ethical Standards

Conflict of Interest

Deyun He declares that she has no conflict of interest. Zhengzong Wu declares that he has no conflict of interest. Bo Cui declares that he has no conflict of interest. Enbo Xu declares that he has no conflict of interest. Zhengyu Jin declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human or animal subjects.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Plant Physiology and Biochemistry, College of Biological SciencesChina Agricultural UniversityBeijingChina
  2. 2.State Key Laboratory of Biobased Material and Green Papermaking, College of Food Science and Engineering, Shandong Academy of SciencesQilu University of TechnologyJinanChina
  3. 3.State Key Laboratory of Food Science and Technology, School of Food Science and TechnologyJiangnan UniversityWuxiChina

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