Food Analytical Methods

, Volume 12, Issue 2, pp 604–611 | Cite as

Size Exclusion Coupled to Reversed Phase Liquid Chromatography for the Characterization of Cranberry Products

  • Inal Bakhytkyzy
  • Oscar Nuñez
  • Javier SaurinaEmail author


A combination of solvent extraction, size exclusion purification, and reversed-phase liquid chromatography with UV absorption and fluorescence detection was here used for the determination of flavan-3-ols and related oligomeric compounds in cranberry products. The method relied on robust, simple, and inexpensive instrumental equipment present in most of routine analytical laboratories. Sample extracts in methanol/water/hydrochloric acid solution (60:39:1, v/v/v) were subjected to size-exclusion chromatography (SEC) using Sephadex LH-20 as the sorbent to separate simple phenolic and oligomeric components. As a novel aspect of this work, the implementation of the SEC process in a reusable packed column was practically and economically highly advantageous. Fractions were analyzed by reversed-phase chromatography using a C18 core-shell analytical column and 0.1% formic acid aqueous solution and methanol as the components of the mobile phase. Chromatograms were recorded by UV absorption spectroscopy at 280 nm and by fluorescence spectroscopy at λex 280 nm/λem 347 nm. The method was applied to the determination of flavan-3-ols in various cranberry samples. Catechin, epicatechin, and procyanidins A2 and B2 were found in different amounts depending on the cranberry varieties.


Cranberry Flavanols Procyanidin A2 Size-exclusion chromatography Liquid chromatography 



The authors gratefully acknowledge the financial support received from Spanish Ministry of Economy and Competitiveness under the projects CTQ2014-65324 and CTQ2015-63968-C2-1-P, and from the Agency for Administration of University and Research Grants (Generalitat de Catalunya, Spain) under the projects 2014 SGR-377 and 2014 SGR-539.

Compliance with Ethical Standards

Conflict of Interest

Inal Bakhytkyzy declares that he has no conflict of interest. Oscar Nuñez declares that he has no conflict of interest. Javier Saurina declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with humans and animals performed by any of the authors.

Informed Consent

Not applicable.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemical Engineering and Analytical ChemistryUniversity of BarcelonaBarcelonaSpain
  2. 2.Research Institute in Food Nutrition and Food SafetyUniversity of BarcelonaBarcelonaSpain
  3. 3.Serra Húnter Fellow, Generalitat de CatalunyaBarcelonaSpain

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