Zhenjiang aromatic vinegar is one of the most popular vinegars in China, which can be produced by either traditional or modern techniques. Aroma profiles of the traditional Zhenjiang aromatic vinegar (TZAV) and the modern Zhenjiang aromatic vinegar (MZAV) at different stages of their aging were analyzed by headspace solid-phase microextraction (HS-SPME) with an aid of DVB/CAR/PDMS fiber combined with gas chromatography-mass spectrometry (GC-MS) and gas chromatography-olfactometry (GC-O). A total of 53 volatile compounds were identified. Among them, 43 aromas were positively identified after comparison with their corresponding authentic compounds. Two volatiles, including isopentyl alcohol and butyl benzoate, were only identified in TZAV, while propiophenone was only identified in MZAV. In addition, some volatiles were found to be generated during the aging period, which included isopentyl alcohol, 3-(methylthio)propyl acetate, and 2-acetoxy-3-butanone in the TZAV samples, and 2-ethyl-1-hexanol, octanoic acid, propiophenone, and dimethyl trisulfide in the MZAV samples. The total amount of volatile compounds in the MZAV was greater than its counterpart TZAV. Moreover, 29 odor-active compounds were recognized by GC-O analysis, while 25 of them were confirmed by their authentic compounds, and their perceived odors included sweet, acid, buttery, floral, herbal, fruit, fresh, pungent, almond, and geraniol notes.
Zhenjiang vinegar Aging Aromas HS-SPME GC-MS GC-O
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The authors would especially like to thank the manufacturer Jiangsu Hengshun Vinegar Co. Ltd., to provide the traditional and modern Zhenjiang vinegar samples, and Mr. Shenghu Zhu in Jiangsu Hengshun Vinegar Co. Ltd., who supplied us the photos for the different steps of both techniques of Zhenjiang aromatic vinegar.
This work was financially supported by the National Key R&D Program of China (2016YFD0400500).
Compliance with Ethical Standards
Conflict of Interest
Sam Al-Dalali declares that he has no conflict of interest. Fuping Zheng declares that he has no conflict of interest. Baoguo Sun declares that he has no conflict of interest. Feng Chen declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
Aceña L, Vera L, Guasch J, Busto O, Mestres M (2011) Chemical characterization of commercial Sherry vinegar aroma by headspace solid-phase microextraction and gas chromatography-olfactometry. J Agric Food Chem 59:4062–4070CrossRefGoogle Scholar
Budak NH, Aykin E, Seydim AC, Greene AK, Guzel-Seydim ZB (2014) Functional properties of vinegar. J Food Sci 79(5):757–764CrossRefGoogle Scholar
Callejón RM, Morales ML, Ferreira ACS, Troncoso AM (2008) Defining the typical aroma of Sherry vinegar: sensory and chemical approach. J Agric Food Chem 56:8086–8095CrossRefGoogle Scholar
Callejón RM, Tesfaye W, Torija MJ, Mas A, Troncoso AM, Morales ML (2009) Volatile compounds in red wine vinegars obtained by submerged and surface acetification in different woods. Food Chem 113:1252–1259CrossRefGoogle Scholar
Cejudo-Bastante C, Durán-Guerrero E, García-Barroso C, Castro-Mejías R (2018) Comparative study of submerged and surface culture acetification process for orange vinegar. J Sci Food Agric 98:1052–1060CrossRefGoogle Scholar
Charles M, Martin B, Ginies C, Etievant P, Coste G, Guichard E (2000) Potent aroma compounds of two red wine vinegars. J Agric Food Chem 48:70–77CrossRefGoogle Scholar
Chen F, Li L, Qu J, Chen C (2009) Cereal vinegars made by solid-state fermentation in China. In: Solieri L, Giudici P (ed) Vinegars of the world. Springer-Verlag, Italia, Chapter 15, pp. 243–259Google Scholar
Chen S, Xu Y, Qian MC (2013a) Aroma characterization of Chinese rice wine by gas chromatography−olfactometry, chemical quantitative analysis, and aroma reconstitution. J Agric Food Chem 61:11295–11302CrossRefGoogle Scholar
Chen T, Gui Q, Shi JJ, Zhang XY, Chen FS (2013b) Analysis of variation of main components during aging process of Shanxi aged vinegar. Acetic Acid Bact 2:31–38Google Scholar
Chinnici F, Guerrero ED, Sonni F, Natali N, Marín RN, Riponi C (2009) Gas chromatography-mass spectrometry (GC-MS) characterization of volatile compounds in quality vinegars with protected European geographical indication. J Agric Food Chem 57:4784–4792CrossRefGoogle Scholar
Fors S (1983) Sensory properties of volatile maillard reaction products and related compounds: a literature review. In: Waller GR, Feather MS (eds) The Maillard reaction in foods and nutrition. American Chemical Society, Washington, D.C., pp 185–286CrossRefGoogle Scholar
Garcia-Parilla MC, Gonzalez GA, Heredia FJ, Troncoso AM (1997) Differentiation of wine vinegars based on phenolic composition. J Agric Food Chem 45:3487–3492CrossRefGoogle Scholar
Guerrero ED, Chinnici F, Natali N, Marín RN, Riponi C (2008) Solid-phase extraction method for determination of volatile compounds in traditional balsamic vinegar. J Sep Sci 31:3030–3036CrossRefGoogle Scholar
Liang J, Xie J, Hou L, Zhao M, Zhao J, Cheng J, Wang S, Sun BG (2016) Aroma constituents in Shanxi aged vinegar before and after aging. J Agric Food Chem 64(40):7597–7605CrossRefGoogle Scholar
Liu H, Sun BG (2018) Effect of fermentation processing on the flavor of baijiu. J Agric Food Chem 66:5425–5432CrossRefGoogle Scholar
Liu J, Gan J, Yu Y, Zhu S, Yin L, Cheng Y (2016) Effect of laboratory-scale decoction on the antioxidative activity of Zhenjiang aromatic vinegar: the contribution of melanoidins. J Functional Foods 21:75–86CrossRefGoogle Scholar
Lu ZM, Xu W, Yu NH, Zhou T, Li GQ, Shi JS, Xu ZH (2011) Recovery of aroma compounds from Zhenjiang aromatic vinegar by supercritical fluid extraction. Int J Food Sci Technol 46:1508–1514CrossRefGoogle Scholar
Mejías RC, Marín RN, Moreno MVG (2002) Optimisation of headspace solid-phase microextraction for analysis of aromatic compounds in vinegar. J Chromatogr A 953:7–15CrossRefGoogle Scholar
Morales ML, González AG, Casas JA, Troncoso AM (2001) Multivariate analysis of commercial and laboratory produced Sherry wine vinegars: influence of acetification and aging. Eur Food Res Technol 212:676–682CrossRefGoogle Scholar
Natera R, Castro R, Garciäa-moreno MDV, Ndez MJH, Garciäa-Barroso C (2003) Chemometric studies of vinegars from different raw materials and processes of production. J Agric Food Chem 51:3345–3351CrossRefGoogle Scholar
Shen ZY (2007) Zhenjiang aromatic vinegar. Hong Kong Medical Press, Hong KongGoogle Scholar
Ubeda C, Callejón RM, Troncoso AM, Moreno-Rojas JM, Peña F, Morales ML (2012) Characterization of odour active compounds in strawberry vinegars. Flavour Fragrance J 27:313–321CrossRefGoogle Scholar
Wang CY, Zhang J, Gui ZZ (2015a) Acetobacter bacteria are found in Zhenjiang vinegar grains. Genet Mol Res 14(2):5054–5064CrossRefGoogle Scholar
Wang ZM, Lu ZM, Yu YJ, Li GQ, Shi JS, Xu ZH (2015b) Batch-to-batch uniformity of bacterial community succession and flavor formation in the fermentation of Zhenjiang aromatic vinegar. Food Microbiol 50:64–69CrossRefGoogle Scholar
Xiao ZB, Dai SP, Niu YW, Yu HY, Zhu JC, Tian HX, Gu YB (2011) Discrimination of Chinese vinegars based on headspace solid-phase microextraction-gas chromatography mass spectrometry of volatile compounds and multivariate analysis. J Food Sci 76:C1125–C1135CrossRefGoogle Scholar
Xiong C, Zheng Y, Xing Y, Chen S, Zeng Y (2015) Discrimination of two kinds of geographical origin protected Chinese vinegars using the characteristics of aroma compounds and multivariate statistical analysis. Food Anal Methods 9(3):768–776CrossRefGoogle Scholar
Xu Y, Wang D, Fan WL, Mu XQ, Chen J (2009) Traditional Chinese biotechnology. In: Tsao G, Ouyang P, Chen J (eds) Biotechnology in China II. Advances in biochemical engineering / biotechnology, vol 122. Springer, Berlin, Heidelberg, pp 189–233Google Scholar
Yu YJ, Lu ZM, Yu NH, Xu W, Li GQ, Shi JS, Xu ZH (2012) HS-SPME/GC-MS and chemometrics for volatile composition of Chinese traditional aromatic vinegar in the Zhenjiang region. J Inst Brew 118:133–141CrossRefGoogle Scholar
Zhou Z, Liua S, Konga X, Jia Z, Hana X, Wu J, Mao J (2017) Elucidation of the aroma compositions of Zhenjiang aromatic vinegar using comprehensive two dimensional gas chromatography coupled to time-of-flight mass spectrometry and gas chromatography-olfactometry. J Chromatogr A 1487:218–226CrossRefGoogle Scholar
Zhu H, Zhu J, Wang LL, Li ZG (2016) Development of a SPMEGC-MS method for the determination of volatile compounds in Shanxi aged vinegar and its analytical characterization by aroma wheel. J Food Sci Technol 53:171–183CrossRefGoogle Scholar