Food Analytical Methods

, Volume 12, Issue 1, pp 246–257 | Cite as

Accuracy and Precision Comparison for Molecular Weight Distribution Assay of Fish Collagen Peptides: a Methodology Study Between Two Gel Permeation Chromatography Columns

  • Xiaoling Lin
  • Yunjun Lu
  • Ting Zhang
  • Ming Liang
  • Yueyan Cen
  • Erdong Yuan
  • Jiaoyan RenEmail author


Two gel permeation chromatography columns, Superdex Peptide HR 10/30 column and TSK G2000 SWXL column, were compared for their accuracy and precision for determining the molecular weight distribution of fish collagen peptides using methodology study. To confirm the results, collagen peptides were subjected to ultrafiltration separation and then the filtrates (MW < 3 kDa) were loaded to MALDI-TOF-MS. Superdex column was consider as the most promising candidate for the determination of small molecular weight peptides (MW < 3 kDa), since it spiked recovery was 101.90% and the RSD was below 1%. As for TSK column, it was more suitable for the assay of large molecular weight peptides (MW > 10 kDa). The spiked recovery was 105.66% and the RSD was below 0.5%. Besides, acetonitrile reagent narrowed the pore size distribution of column packing materials, which made it more inaccurate for the analysis of smaller molecular weight peptides.


Fish collagen peptides Molecular weight distribution (MW distribution) Gel permeation chromatography (GPC) Centrifugal ultrafiltration MALDI-TOF-MS 



This study is supported by the National Natural Science Foundation of China [No. 31671804], Guangdong Special Funding for Outstanding Young Scholars [2014TQ01N645], Guangdong Science and Technology Planning Project [2017B090901063 and 2015B020230001], Science and Technology Program of Guangzhou [201604020047], the Fundamental Research Funds for the Central Universities [017ZD079], and the 111 Project [B17018].

Compliance with Ethical Standards

Conflict of Interest

Xiaoling Lin declares that she has no conflict of interest. Yunjun Lu declares that she has no conflict of interest. Ting Zhang declares that she has no conflict of interest. Ming Liang declares that she has no conflict of interest. Yueyan Cen declares that she has no conflict of interest. Erdong Yuan declares that she has no conflict of interest. Jiaoyan Ren declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human or animal subjects by any of the authors.

Informed Consent

Not applicable.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Xiaoling Lin
    • 1
  • Yunjun Lu
    • 1
  • Ting Zhang
    • 2
  • Ming Liang
    • 2
  • Yueyan Cen
    • 1
  • Erdong Yuan
    • 1
    • 3
    • 4
  • Jiaoyan Ren
    • 1
    • 3
    • 4
    Email author
  1. 1.School of Food Sciences and EngineeringSouth China University of TechnologyGuangzhouChina
  2. 2.Infinitus (China) Ltd.GuangzhouChina
  3. 3.Sino-Singapore International Joint Research InstituteGuangzhou Knowledge CityChina
  4. 4.Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111Center)GuangzhouChina

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