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Recombinase Polymerase Amplification Combined with Unmodified Gold Nanoparticles for Salmonella Detection in Milk

  • Zhi-guang Chen
  • Hai-xia Zhong
  • Huan Luo
  • Ren-yu Zhang
  • Jun-rong Huang
Article
  • 107 Downloads

Abstract

Salmonella is one of the most common pathogenic bacterium, which causes food spoilage and human diseases. The national standard detection method (GB4789.4) and other fast detection methods for Salmonella cannot satisfy the requirements in the present climate because of their inefficiency, low accuracy, high equipment requirements, or high cost. In this study, we combine isothermal recombinase polymerase amplification (RPA) with unmodified gold nanoparticles (AuNPs) to detect Salmonella in milk. A rapid, effective, visualized, and low-cost RPA-AuNP assay was developed, with a detection limit of 50 CFU for milk samples after enrichment for 6 h or 1 pg for DNA within 15 min at 37 °C using simple water bath equipment. Furthermore, it exhibited no cross-reactions with other pathogens by using highly specific region of invA of Salmonella as the target detection gene. And it may apply to the local food inspection and detection center whose equipment conditions are poor.

Keywords

RPA AuNPs Food detection Salmonella 

Notes

Compliance with Ethical Standards

Ethical Approval

Not applicable.

Informed Consent

Not applicable.

Conflict of Interest

Zhi-guang Chen declares that he has no conflict of interest. Hai-xia Zhong declares that she has no conflict of interest. Huan Luo declares that she has no conflict of interest. Ren-yu Zhang declares that she has no conflict of interest. Jun-rong Huang declares that she has no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Zhi-guang Chen
    • 1
    • 2
  • Hai-xia Zhong
    • 2
  • Huan Luo
    • 2
  • Ren-yu Zhang
    • 2
  • Jun-rong Huang
    • 1
  1. 1.School of Food and Biological EngineeringShaanxi University of Science and TechnologyXi’anChina
  2. 2.Department of Agricultural TechnologyNeijiang Vocational and Technical CollegeNeijiangChina

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