Isolation and Identification of Metabolites in Chinese Northeast Potato (Solanum tuberosum L.) Tubers Using Gas Chromatography-Mass Spectrometry
The gas chromatography coupled with mass spectrometry was established for the isolation and identification of the 65 compounds and main compounds including sugar, sugar alcohols, dimeric and trimeric saccharides, amino acid, and organic acid, in Chinese northeast potato (Solanum tuberosum L.) tubers. Polar metabolites were extracted and separated from potato tubers of the four kinds of potatoes in Chinese northeast with 80% methanol, and derived with N, O-Bis (trimethylsilyl) trifluoroacetamide. Then, the analytical performances of the proposed method were evaluated. When the reproducibility was evaluated, the relative standard deviations (RSDs) of both peak count of 90.4% metabolites and peak areas of 91.4% metabolites were lower than 30%. When the stability was evaluated, the RSDs of both peak count of 76.7% metabolites and cumulative peak areas of 92.5% metabolites were lower than 30%. In contrast to the databases, some of the primary and secondary metabolites were identified. The established method is expected to be useful for the metabolomic studies of potato.
KeywordsMetabolites Potato tuber GC-MS Identification Chinese northeast
This study was funded by the Natural Science Foundation of Heilongjiang Province of China (Grant number: B2015010), Project funded by the China Postdoctoral Science Foundation (Grant number:2017 M611413), Special Funding of Postdoctoral in Heilongjiang BaYi Agricultural University, and Program for Young Scholars with Creative Talents in Heilongjiang BaYi Agricultural University (Grant number: CXRC2017011).
Compliance with Ethical Standards
Conflict of Interest
Liyuan Zhang declares that she has no conflict of interest. Yingbo Yu declares that she has no conflict of interest. Changyuan Wang declares that he has no conflict of interest. Dongjie Zhang declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
Informed consent was obtained from all individual participants included in the study.
- Evans AM, DeHaven CD, Barrett T, Mitchell M, Milgram E (2009) Integrated, nontargeted ultrahigh performance liquid chromatography/electrospray ionization tandem mass spectrometry platform for the identification and relative quantification of the small-molecule complement of biological systems. Anal Chem 81:6656–6667CrossRefGoogle Scholar
- He XQ, Tan DG, Sun XP, Peng M, Zhang JM (2012) Isolation and identification of polar metabolites in cassava leaf using GC-MS method. Chin J Trop Crops 22:422–426Google Scholar
- Soininen P, Kangas AJ, Würtz P, Tukiainen T, Tynkkynen T, Laatikainen RJ, Järvelin MR, Kähönen M, Lehtimäki T, Viilari J, Raitakari OT, Savolainen MJ, Korpela MA (2009) High-throughput serum NMR metabonomics for cost-effective holistic studies on systemic metabolism. Analyst 134:1781–1785CrossRefGoogle Scholar