Food Analytical Methods

, Volume 12, Issue 1, pp 51–58 | Cite as

Isolation and Identification of Metabolites in Chinese Northeast Potato (Solanum tuberosum L.) Tubers Using Gas Chromatography-Mass Spectrometry

  • Liyuan Zhang
  • Yingbo Yu
  • Changyuan WangEmail author
  • Dongjie ZhangEmail author


The gas chromatography coupled with mass spectrometry was established for the isolation and identification of the 65 compounds and main compounds including sugar, sugar alcohols, dimeric and trimeric saccharides, amino acid, and organic acid, in Chinese northeast potato (Solanum tuberosum L.) tubers. Polar metabolites were extracted and separated from potato tubers of the four kinds of potatoes in Chinese northeast with 80% methanol, and derived with N, O-Bis (trimethylsilyl) trifluoroacetamide. Then, the analytical performances of the proposed method were evaluated. When the reproducibility was evaluated, the relative standard deviations (RSDs) of both peak count of 90.4% metabolites and peak areas of 91.4% metabolites were lower than 30%. When the stability was evaluated, the RSDs of both peak count of 76.7% metabolites and cumulative peak areas of 92.5% metabolites were lower than 30%. In contrast to the databases, some of the primary and secondary metabolites were identified. The established method is expected to be useful for the metabolomic studies of potato.


Metabolites Potato tuber GC-MS Identification Chinese northeast 



This study was funded by the Natural Science Foundation of Heilongjiang Province of China (Grant number: B2015010), Project funded by the China Postdoctoral Science Foundation (Grant number:2017 M611413), Special Funding of Postdoctoral in Heilongjiang BaYi Agricultural University, and Program for Young Scholars with Creative Talents in Heilongjiang BaYi Agricultural University (Grant number: CXRC2017011).

Compliance with Ethical Standards

Conflict of Interest

Liyuan Zhang declares that she has no conflict of interest. Yingbo Yu declares that she has no conflict of interest. Changyuan Wang declares that he has no conflict of interest. Dongjie Zhang declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

12161_2018_1336_MOESM1_ESM.doc (94.4 mb)
ESM 1 (DOC 96644 kb)


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of FoodHeilongjiang Bayi Agricultural UniversityDaqingPeople’s Republic of China
  2. 2.Key Laboratory of Agro-Products Processing and Quality Safety of Heilongjiang provinceDaqingPeople’s Republic of China

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