Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents

  • Vesna Vukojević
  • Sladjana Djurdjić
  • Ľubomír Švorc
  • Tanja Ćirković Veličković
  • Jelena Mutić
  • Dalibor M. Stanković
Article
  • 27 Downloads

Abstract

Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 μM was estimated, with limit of detection of 0.7 μM, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.

Keywords

Ergosterol Boron-doped diamond electrode Mushrooms 

Notes

Compliance with Ethical Standards

Conflict of Interest

Vesna Vukojević declares that she has no conflict of interest. Sladjana Djurdjić declares that she has no conflict of interest. Ľubomír Švorc declares that he has no conflict of interest. Tanja Ćirković-Veličković declares that she has no conflict of interest. Jelena Mutić declares that she has no conflict of interest. Dalibor M. Stanković declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human or animal subjects performed by any of the authors.

Informed Consent

Informed consent is not applicable for the nature of this study.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Vesna Vukojević
    • 1
  • Sladjana Djurdjić
    • 1
  • Ľubomír Švorc
    • 2
  • Tanja Ćirković Veličković
    • 3
    • 4
  • Jelena Mutić
    • 3
    • 4
  • Dalibor M. Stanković
    • 1
    • 5
  1. 1.Innovation Center of the Faculty of ChemistryUniversity of BelgradeBelgradeSerbia
  2. 2.Institute of Analytical Chemistry, Faculty of Chemical and Food TechnologySlovak University of Technology in BratislavaBratislavaSlovak Republic
  3. 3.Faculty of ChemistryUniversity of BelgradeBelgradeSerbia
  4. 4.Ghent University Global CampusIncheonSouth Korea
  5. 5.The “Vinča” Institute of Nuclear SciencesUniversity of BelgradeBelgradeSerbia

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