Multiplex Flow Cytometric Immunoassays for High-Throughput Screening of Multiple Mycotoxin Residues in Milk
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Microsphere-based immunoassays involving flow cytometry have recently gained popularity for use in protein detection and infectious disease diagnosis due to their simple assay format and capacity for multiplexed analysis. In this work, four mycotoxins, including aflatoxin M1 (AFM1), ochratoxin A (OTA), deoxynivalenol (DON), and fumonisin B1 (FB1), were selected as model analytes. A quadplex flow cytometric immunoassay (FCIA) was developed for detecting multiple mycotoxin residues in milk. The optimized quadplex FCIA exhibited satisfactory compatibility with the multiple mycotoxin residue analysis, with limits of detection (LODs) of 0.045 μg L−1 for AFM1, 0.94 μg L−1 for OTA, 7.48 μg L−1 for DON, and 2.45 μg L−1 for FB1. The recoveries of the target mycotoxins from spiked milk were 76–95%, with a relative standard deviation of less than 13.4%. Compared to traditional ELISA formats, the FCIA provided lower detection limits for multiple mycotoxin detection.
KeywordsMycotoxin Multi-analyte residue Flow cytometric immunoassay Milk Food safety
This work was supported by the National Natural Science Foundation of China (No. 31602103); the Science and Technology Planning Project of Guangdong Province, China (Nos. 2016A020210055 and 2017B020207009); the Shenzhen Basic Research Project (Nos. JCYJ20160307114724751 and JCYJ20170817095240632); and the Shenzhen Sanming Project of Medicine and Health (No. SZSM201611068).
Compliance with Ethical Standards
Conflict of Interest
Jianwen Qu declares that he has no conflict of interest. Huijuan Xie declares that he has no conflict of interest. Shuying Zhang declares that he has no conflict of interest. Pengjie Luo declares that he has no conflict of interest. Ping Guo declares that he has no conflict of interest. Yuebin Ke declares that he has no conflict of interest. Junyu Zhuang declares that he has no conflict of interest. Fangmei Zhou declares that he has no conflict of interest. Wenxiao Jiang declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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