Development of an Upconversion Luminescence Nanoparticles–Based Immunochromatographic Assay for the Rapid Detection of Dexamethasone in Animal Tissue
- 135 Downloads
Dexamethasone has been illegally used as a growth-promoting agent to obtain an economic benefit from increased muscle development. An immunochromatographic assay was developed for the quantitative detection of dexamethasone using a probe based on upconversion luminescence nanoparticles. Lanthanide-doped upconversion nanoparticles were synthesized in a system comprising water and ethylene glycol. Various parameters, including the immunoreaction time, the structure of the coating hapten, and the type and concentration of surfactant, were optimized. Based on the optimal conditions, the assay exhibits good dynamic linear detection in the range of 0.05–5 ng/mL with an IC50 of 0.58 ng/mL. The detection limits for dexamethasone in tissue are 0.3 μg/kg. The assay worked well using a 30% methanol extraction solution, giving an average recovery of 84.5%. The assay strip can quantitate on-site using a portable upconversion luminescence reader with a cumulative analysis time of only 10 min, and its fluorescence decay after 6 months is < 25%.
KeywordsUpconversion luminescence nanoparticles Dexamethasone Immunochromatography Quantitative
Compliance with Ethical Standards
The work was financially supported by the Shenzhen science and technology bureau basic research project (JCYJ20170817162119873) and the State Administration for Quality Supervision and Inspection and Quarantine research project of China (2017QK095).
Conflict of Interest
Shiwei Zhang declares that he has no conflict of interest. Tianqi Yao declares that he has no conflict of interest. Shifeng Wang declares that he has no conflict of interest. Ronghu Feng declares that he has no conflict of interest. Liqiong Chen declares that he has no conflict of interest. Vivian Zhu declares that she has no conflict of interest. Guiping Hu declares that he has no conflict of interest. Heng Zhang declares that he has no conflict of interest. Guowu Yang declares that he has no conflict of interest.
This article does not contain any studies with human subjects. All animal experiments that described in the present study were performed in adherence to Shenzhen Academy of Metrology and Quality Inspection animal experiment center guidelines and approved by Animal Ethics Committee.
A statement regarding informed consent is not applicable.
- Arcelloni C, Lanzi R, Pedercini S, Molteni G, Fermo I, Pontiroli A, Paroni R (2001) High-performance liquid chromatographic determination of diclofenac in human plasma after solid-phase extraction. J Chromatogr B Biomed Sci Appl 763(1-2):195–200. https://doi.org/10.1016/S0378-4347(01)00383-8 CrossRefGoogle Scholar
- Bagnati R, Ramazza V, Zucchi M, Simonella A, Leone F, Bellini A, Fanelli R (1996) Analysis of dexamethasone and betamethasone in bovine urine by purification with an “online” immunoaffinity chromatography–high-performance liquid chromatography system and determination by gas chromatography–mass spectrometry. Anal Biochem 235(2):119–126. https://doi.org/10.1006/abio.1996.0103 CrossRefGoogle Scholar
- Bhargava D, Deshpande A, Thomas S, Sharma Y, Khare P, Sahu SK, Sreekumar K (2016) High performance liquid chromatography determination of dexamethasone in plasma to evaluate its systemic absorption following intra-space pterygomandibular injection of twin-mix (mixture of 2% lignocaine with 1:200,000 epinephrine and 4 mg dexamethasone): randomized control trial. Oral Maxillofac Surg 20(3):259–264. https://doi.org/10.1007/s10006-016-0564-3 CrossRefGoogle Scholar
- Boyer JC, Vetrone F, Cuccia LA, Capobianco JA (2006) Synthesis of colloidal upconverting NaYF4 nanocrystals doped with Er3+, Yb3+ and Tm3+, Yb3+ via thermal decomposition of lanthanide trifluoroacetate precursors. J Am Chem Soc 128(23):7444–7445. https://doi.org/10.1021/ja061848b CrossRefGoogle Scholar
- Chen D, Tao Y, Liu Z, Zhang H, Liu Z, Wang Y, Yuan Z (2011) Development of a liquid chromatography-tandem mass spectrometry with pressurized liquid extraction for determination of glucocorticoid residues in edible tissues. J Chromatogr B 879(2):174–180. https://doi.org/10.1016/j.jchromb.2010.11.039 CrossRefGoogle Scholar
- Commission C A (2009). Compendium of methods of analysis identified as suitable to support Codex MRLs. 32nd session.Google Scholar
- Huetos Hidalgo O, Jiménez López M, Ajenjo Carazo E, San Andrés Larrea M, Reuvers TBA (2003) Determination of dexamethasone in urine by gas chromatography with negative chemical ionization mass spectrometry. J Chromatogr B 788(1):137–146. https://doi.org/10.1016/S1570-0232(02)01039-5 CrossRefGoogle Scholar
- Pimpitak U, Putong S, Komolpis K, Petsom A, Palaga T (2009) Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for detection of the furaltadone metabolite, AMOZ, in fortified shrimp samples. Food Chem 116(3):785–791. https://doi.org/10.1016/j.foodchem.2009.03.028 CrossRefGoogle Scholar
- Ray JA, Kushnir MM, Rockwood AL, Meikle AW (2011) Analysis of cortisol, cortisone and dexamethasone in human serum using liquid chromatography tandem mass spectrometry and assessment of cortisol: cortisone ratios in patients with impaired kidney function. Clin Chim Acta 412(13-14):1221–1228. https://doi.org/10.1016/j.cca.2011.03.016 CrossRefGoogle Scholar
- Sami M, Farzaneh N, Mohri M, Seifi HA (2015) Effects of dexamethasone and insulin alone or in combination on energy and protein metabolism indicators and milk production in dairy cows in early lactation – a randomized controlled trial. PLoS One 10(9):e0139276. https://doi.org/10.1371/journal.pone.0139276 CrossRefGoogle Scholar
- Vincenti M, Girolami F, Capra P, Pazzi M, Carletti M, Gardini G, Nebbia C (2009) Study of Dexamethasone urinary excretion profile in cattle by LC− MS/MS: comparison between therapeutic and growth-promoting administration. J Agric Food Chem 57(4):1299–1306. https://doi.org/10.1021/jf803465d CrossRefGoogle Scholar
- Zhao B & Li Y (2018) Facile synthesis of near-infrared-excited NaYF4: Yb3+, Tm3+ nanoparticles for label-free detection of dopamine in biological fluids, Talanta 179:478–484. https://doi.org/10.1016/j.talanta.2017.11.042