Extensive Validation of Gas Chromatographic Method for Determination of Esters in Wines Using Monolithic Materials as Preconcentration Step
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A comprehensive validation of a quantitative method is a critical issue for food analysis, which requires reliable estimation of uncertainties influencing measurements. Esters as important contributors to fruity aroma generally affect quality of wine and its organoleptic features. In this study, a full validation of the method developed for quantification of esters with uncommon silica monolithic discs and gas chromatographic analysis is presented. Two types of sorbent modification and a few desorption solvents were tested for ester removal from wine samples. Uncertainty contributions from mass balance tolerance, purity of chemicals, volume measurements, calibration curve deviation and extraction procedure were included in the calculation of the combined uncertainty. The expanded uncertainty of ester determination ranged from 15 to 37% and was mainly affected by uncertainty of calibration curve. The proposed method provided a limit of detection and a limit of quantification of 0.005–0.168 μg/L and 0.018–0.560 μg/L, respectively. Additional measurements revealed a recovery range of 70–111%, average reproducibility of instrument response less than 4%, and repeatability of the method below 16% for most of esters.
KeywordsValidation Esters Monolithic material Wine GC-FID
This work was supported under kind support of Slovak Research and Development Agency under contract number APVV-15-0333
Research Involving Human Participants and/or Animals or Ethical Approval
This article does not contain studies with human participants or animals performed by any of the authors.
Compliance with Ethical Standards
Conflict of Interest
Olga Vyviurska declares that she has no conflict of interest. Roman Gorovenko declares that he has no conflict of interest. Veronika Panáková declares that she has no conflict of interest. Ivan Špánik declares that he has no conflict of interest.
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