A rapid, simple, and efficient method was described for the simultaneous determination of three common antibiotic residues including erythromycin, tetracycline, and chloramphenicol in raw milk. A molecular imprinting technique combined with solid-phase extraction (SPE) was used to pretreat the test samples and then simultaneously detected with HPLC. The whole process, only including one step of pretreatment, was able to detect the entire target molecule using specific enrichment and analysis method. The detection was verified by comparison with Chinese standard methods of GB/T 22988-2008, GB/T 22990-2008, and GB/T 29688-2013. The testing lines, accuracy, reproducibility, specificity, and recoveries were evaluated, and calculated values were in line with established standards. The recoveries of erythromycin, tetracycline, and chloramphenicol were 77.82~87.08, 81.02~88.17, and 72.94~83.57%, respectively. By using of this novel solid-phase extraction substrate, the detection limits for erythromycin, tetracycline, and chloramphenicol were 10, 20, and 10 μg kg−1, respectively. The method was suitable for routine analysis, and the experimental procedure was simplified, with the detection time greatly reduced. This method showed broad application prospects for the detection of antibiotic residues in raw milk.
Simultaneous detection Molecular imprinting Solid-phase extraction Antibiotics Raw milk
Compliance with Ethical Standards
This article is supported by the the Jiangsu Agriculture Science and Technology Innovation Fund (JASTIF), CX(16)1028, the National Key Technology R&D Program in the 12th Five-Year Plan of China (No. 2015BAD16B01), National Key Technology R&D Program in the 12th Five-Year Plan of Suzhou (SS201440), and the program of the “Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.”
Conflict of Interest
Yunfei Xie declares that she has no conflict of conflict of interest. Qi Hu declares that she has no conflict of interest. Mengyao Zhao declares that she has no conflict of interest. Yuliang Cheng declares that he has no conflict of interest. Yahui Guo declares that he has no conflict of interest. He Qian declares that she has no conflict of interest. Weirong Yao declares that she has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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