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Food Analytical Methods

, Volume 10, Issue 12, pp 4062–4067 | Cite as

Examination of the Varied and Changing Ethanol Content of Commercial Kombucha Products

  • Mohsen Talebi
  • Lillian A. Frink
  • Rahul A. Patil
  • Daniel W. ArmstrongEmail author
Article

Abstract

Kombucha is a fermented beverage made by mixing tea and sugar with bacteria and yeast. When kombucha products contain higher than 0.5% (v/v) alcohol, the legal limit for non-alcoholic drinks, they are classified as alcoholic beverages and are subject to relevant federal and state regulations. An efficient headspace gas chromatography technique utilizing an ionic liquid stationary phase is developed to accurately determine the ethanol content in 18 commercial kombucha samples. The range of ethanol in these products was 1.12–2.00% (v/v). The ethanol concentration of two batches of kombucha was analyzed over a period of 60 days under two different conditions. A significant increase in ethanol content of these samples was observed at 4 and 22 °C. The method accuracy was validated by analyzing 3 NIST ethanol-water standard reference solutions.

Keywords

Kombucha Ethanol analysis Ionic liquid column Headspace gas chromatography 

Notes

Acknowledgements

The authors gratefully acknowledge financial support from the Welch Foundation (Y0026). Moreover, we would like to thank Shimadzu Scientific Instruments for instrumental support.

Compliance with Ethical Standards

Conflict of Interest

Mohsen Talebi declares that he has no conflict of interest. Lilian A. Frink declares that she has no conflict of interest. Rahul A. Patil declares that he has no conflict of interest. Daniel W. Armstrong declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human or animal subjects.

Informed Consent

Not applicable.

Supplementary material

12161_2017_980_MOESM1_ESM.docx (243 kb)
ESM 1 (DOCX 242 kb)

References

  1. Alcohol and Tobacco Tax and Trade Bureau (2016) Kombucha information and resources. https://www.ttb.gov/kombucha/ Accessed 25 April 2017
  2. Blanc PJ (1996) Characterization of the tea fungus metabolites. Biotechnol Lett 18(2):139–142CrossRefGoogle Scholar
  3. Cheng C, Liu S, Mueller BJ, Yan Z (2010) A generic static headspace gas chromatography method for determination of residual solvents in drug substance. J Chromatogr A 1217(41):6413–6421CrossRefGoogle Scholar
  4. Dufresne C, Farnworth E (2000) Tea, kombucha, and health: a review. Food Res Int 33(6):409–421CrossRefGoogle Scholar
  5. Frink LA, Armstrong DW (2016a) The utilisation of two detectors for the determination of water in honey using headspace gas chromatography. Food Chem 205:23–27CrossRefGoogle Scholar
  6. Frink LA, Armstrong DW (2016b) Water determination in solid pharmaceutical products utilizing ionic liquids and headspace gas chromatography. J Pharm Sci 105(8):2288–2292CrossRefGoogle Scholar
  7. Frink LA, Armstrong DW (2016c) Determination of trace water content in petroleum and petroleum products. Anal Chem 88(16):8194–8201CrossRefGoogle Scholar
  8. Frink LA, Weatherly CA, Armstrong DW (2014) Water determination in active pharmaceutical ingredients using ionic liquid headspace gas chromatography and two different detection protocols. J Pharm Biomed Anal 94:111–117CrossRefGoogle Scholar
  9. Goh W, Rosma A, Kaur B, Fazilah A, Karim A, Bhat R (2012) Fermentation of black tea broth (Kombucha): I. Effects of sucrose concentration and fermentation time on the yield of microbial cellulose. Int Food Res J 19(1):109–117Google Scholar
  10. Greenwalt C, Ledford R, Steinkraus K (1998) Determination and characterization of the antimicrobial activity of the fermented tea kombucha. LWT-Food Sci Technol 31(3):291–296CrossRefGoogle Scholar
  11. Greenwalt C, Steinkraus K, Ledford R (2000) Kombucha, the fermented tea: microbiology, composition, and claimed health effects. J Food Prot 63(7):976–981CrossRefGoogle Scholar
  12. Huang K, Han X, Zhang X, Armstrong DW (2007) PEG-linked geminal dicationic ionic liquids as selective, high-stability gas chromatographic stationary phases. Anal Bioanal Chem 389(7–8):2265–2275CrossRefGoogle Scholar
  13. Ibañez E, Cifuentes A (2001) New analytical techniques in food science. Crit Rev Food Sci Nutr 41(6):413–450CrossRefGoogle Scholar
  14. Jayabalan R, Marimuthu S, Thangaraj P, Sathishkumar M, Binupriya AR, Swaminathan K, Yun SE (2008) Preservation of kombucha tea: effect of temperature on tea components and free radical scavenging properties. J Agric Food Chem 56(19):9064–9071CrossRefGoogle Scholar
  15. Jayabalan R, Malini K, Sathishkumar M, Swaminathan K, Yun S (2010) Biochemical characteristics of tea fungus produced during kombucha fermentation. Food Sci Biotechnol 19(3):843–847CrossRefGoogle Scholar
  16. Jayabalan R, Malbaša RV, Lončar ES, Vitas JS, Sathishkumar M (2014) A review on kombucha tea: microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus. Compr Rev Food Sci Food Saf 13(4):538–550CrossRefGoogle Scholar
  17. Jeleń H, Gracka A, Myśków B (2017) Static headspace extraction with compounds trapping for the analysis of volatile lipid oxidation products. Food Anal Methods. doi: 10.1007/s12161-017-0838-x
  18. Kolb B, Ettre LS (2006) Static headspace-gas chromatography: theory and practice. Wiley, HobokenCrossRefGoogle Scholar
  19. Li H, Chai X, Deng Y, Zhan H, Fu S (2009) Rapid determination of ethanol in fermentation liquor by full evaporation headspace gas chromatography. J Chromatogr A 1216(1):169–172CrossRefGoogle Scholar
  20. Liu M, Li H, Zhan H (2014) A novel method for the determination of the ethanol content in soy sauce by full evaporation headspace gas chromatography. Food Anal Methods 7(5):1043–1046CrossRefGoogle Scholar
  21. Mason M (1983) Ethanol determination in wine with an immobilized enzyme electrode. Am J Enol Vitic 34(3):173–175Google Scholar
  22. Nummer BA (2013) Kombucha brewing under the Food and Drug Administration model food code: risk analysis and processing guidance. J Environ Health 76(4):8–12Google Scholar
  23. Reiss J (1994) Influence of different sugars on the metabolism of the tea fungus. Z Lebensm Unters Forsch 198(3):258–261CrossRefGoogle Scholar
  24. Reva ON, Zaets IE, Ovcharenko LP, Kukharenko OE, Shpylova SP, Podolich OV, de Vera J, Kozyrovska NO (2015) Metabarcoding of the kombucha microbial community grown in different microenvironments. AMB Express 5(1):35CrossRefGoogle Scholar
  25. Sievers M, Lanini C, Weber A, Schuler-Schmid U, Teuber M (1995) Microbiology and fermentation balance in a kombucha beverage obtained from a tea fungus fermentation. Syst Appl Microbiol 18(4):590–594CrossRefGoogle Scholar
  26. Snow NH, Bullock G (2010) Novel techniques for enhancing sensitivity in static headspace extraction-gas chromatography. J Chromatogr A 1217(16):2726–2735CrossRefGoogle Scholar
  27. Sreeramulu G, Zhu Y, Knol W (2000) Kombucha fermentation and its antimicrobial activity. J Agric Food Chem 48(6):2589–2594CrossRefGoogle Scholar
  28. Vīna I, Semjonovs P, Linde R, Denina I (2014) Current evidence on physiological activity and expected health effects of kombucha fermented beverage. J Med Food 17(2):179–188CrossRefGoogle Scholar
  29. Weatherly CA, Woods RM, Armstrong DW (2014) Rapid analysis of ethanol and water in commercial products using ionic liquid capillary gas chromatography with thermal conductivity detection and/or barrier discharge ionization detection. J Agric Food Chem 62(8):1832–1838CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Mohsen Talebi
    • 1
  • Lillian A. Frink
    • 1
  • Rahul A. Patil
    • 1
  • Daniel W. Armstrong
    • 1
    Email author
  1. 1.Department of Chemistry and BiochemistryUniversity of Texas at ArlingtonArlingtonUSA

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