Food Analytical Methods

, Volume 10, Issue 11, pp 3607–3614 | Cite as

Determination of Enrofloxacin in Milk Samples Using Silver Nanoparticle Enhanced Terbium-Sensitized Fluorescence Method

  • Saba Ershadi
  • Abolghasem Jouyban
  • Ali ShayanfarEmail author


Terbium-sensitized fluorescence is one of the simple and sensitive methods for quantification of drugs in biological and food samples. Silver nanoparticles (AgNPs) can enhance the intensity of terbium-sensitized fluorescence and provide a more sensitive method. Enrofloxacin (ENR) is one of the common antibiotics, and the determination of its residue in animal products is recommended because of its problems for human health. Various parameters affecting the fluorescence intensity were optimized using response surface methodology. Then, the optimized parameters were applied to the validation of method for ENR quantification in milk samples. The linearity of the method was from 0.050 to 0.60 mg/L, and the limit of detection (LOD) and limit of quantification (LOQ) of the method were 0.021 to 0.069 mg/L, respectively. The accuracy, precision, and selectivity studies of the developed method give reasonable results. These data showed that the established analysis approach is a simple and sensitive technique for monitoring of ENR in milk samples.


Enrofloxacin Experimental design Silver nanoparticles Terbium-sensitized Fluorescence 



This study was funded by the Vice Chancellor for Research of Tabriz University of Medical Sciences, Tabriz, Iran, for a partial financial support, and it is a part of S. Ershadi’s Pharm.D thesis (No. 3899) registered at Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

Compliance with Ethical Standards

Conflict of Interest

Saba Ershadi declares that he has no conflict of interest. Abolghasem Jouyban declares that he has no conflict of interest. Ali Shayanfar declares that he has no conflict of interest.

Ethical Approval

Not applicable

Informed Consent

Not applicable


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Liver and Gastrointestinal Diseases Research CenterTabriz University of Medical SciencesTabrizIran
  2. 2.Faculty of Pharmacy and Student Research CommitteeTabriz University of Medical SciencesTabrizIran
  3. 3.Pharmaceutical Analysis Research Center and Faculty of PharmacyTabriz University of Medical SciencesTabrizIran
  4. 4.Drug Applied Research Center and Faculty of PharmacyTabriz University of Medical SciencesTabrizIran

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