Food Analytical Methods

, Volume 10, Issue 7, pp 2549–2565 | Cite as

An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens

  • Omar MukamaEmail author
  • Jean Paul Sinumvayo
  • Muhammad Shamoon
  • Muhammad Shoaib
  • Henriette Mushimiyimana
  • Waseem Safdar
  • Leo Bemena
  • Peter Rwibasira
  • Samson Mugisha
  • Zhouping WangEmail author


Foodborne ailments constitute a public health challenge and pose an incredible economic burden in healthcare system around the globe. This dilemma has urged authorities and other entities working in field of food quality control and supply chain to play a pivotal role in ensuring food safety. Analytical strategies have been developed using numerous systematic evolution of ligands by exponential enrichment (SELEX) methods to assure food safety. High-affinity and high-sensitivity ssDNA and RNA aptamers against pathogens have emerged as a novel strategy, as compared to the more resource-demanding and complicated biochemical test-based approaches. Thus, this review aims to focus on some methods used in the selection of specific bare, modified, and conjugated aptamers and on the further analysis of selected aptamers using flow cytometer or post-SELEX modifications for enhanced detection of frequently diagnosed foodborne bacteria such as Bacillus sp., Campylobacter jejuni, Escherichia sp., Salmonella sp., Staphylococcus aureus, Shigella sp., Listeria monocytogenes, and Streptococcus pyogenes and/or targeting their cell components towards attaining fast, sensitive, and selective methods for the detection of pathogens in food(s) or other sources.


Aptamers Modified SELEX Foodborne pathogens Food safety 





American Type Culture Collection


Aptamer fluorescent labeling and magnetic beads immobilization


Aptamer-magnetic bead-electrochemiluminescence


Capillary electrophoresis–SELEX


Centers for Disease Control and Prevention


Deoxyribonucleoside triphosphate 4-propynylpyrrole-2-carbaldehyde


Diol-modified 2-nitro-4-propynylpyrrole


DNA capture element


Enzyme-linked aptamer sedimentation assay


European Centre for Disease Prevention and Control


Fluorescein isothiocyannate


Fluorescence resonance energy transfer


Fluorescence-activated cell sorting




Hydrophobic base 7-(2-thienyl) imidazo[4,5-b]pyridine


Molecular recognition elements


Non-equilibrium capillary electrophoresis of equilibrium mixtures


Outer membrane proteins


Quantum dot


Systematic evolution of ligands by exponential enrichment


Staphylococcus aureus enterotoxin A


Staphylococcus aureus enterotoxin B


Surface-enhanced Raman spectroscopy


Target expressed on cell surface-SELEX


Up conversion nanoparticles




Compliance with Ethical Standards

Conflict of Interest

Omar Mukama declares that he has no conflict of interest. Jean Paul Sinumvayo declares that he has no conflict of interest. Muhammad Shamoon declares that he has no conflict of interest. Muhammad Shoaib declares that he has no conflict of interest. Henriette Mushimiyimana declares that he has no conflict of interest. Waseem Safdar declares that he has no conflict of interest. Leo Bemena declares that he has no conflict of interest. Peter Rwibasira declares that he has no conflict of interest. Samson Mugisha declares that he has no conflict of interest. Zhouping Wang declares that he has no conflict of interest.

Informed Consent

Not applicable. This article does not contain any studies with human or animal subjects.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Omar Mukama
    • 1
    • 2
    Email author
  • Jean Paul Sinumvayo
    • 1
  • Muhammad Shamoon
    • 3
  • Muhammad Shoaib
    • 3
  • Henriette Mushimiyimana
    • 1
  • Waseem Safdar
    • 3
  • Leo Bemena
    • 1
  • Peter Rwibasira
    • 2
  • Samson Mugisha
    • 1
  • Zhouping Wang
    • 3
    Email author
  1. 1.Key Laboratory of Carbohydrate Chemistry and Biotechnology, School of BiotechnologyJiangnan UniversityWuxiPeople’s Republic of China
  2. 2.Department of Applied BiologyCollege of Science and Technology University of RwandaKigaliRwanda
  3. 3.State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and NutritionJiangnan UniversityWuxiPeople’s Republic of China

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