Food Analytical Methods

, Volume 10, Issue 5, pp 1568–1574 | Cite as

The Use of the PHOTOCHEM Device in Evaluation of Antioxidant Activity of Polish Honey

  • Monika Wesołowska
  • Małgorzata Dżugan


Forty samples of varietal Polish honeys were investigated in order to assess their antioxidant capacity by photochemiluminescence (PCL) methods using standard ACW and ACL kits. The reference methods, FRAP and DPPH, were used. The total phenolics content (TPC) by Folin-Ciocalteau method was determined. The antioxidant properties of honeys as well as TPC were mainly dependent on honey botanical origin. The highest phenolic content and antioxidant activity for dark honeys, while the lowest for white honeys, were found. Moreover, the results obtained by used analytical methods were comparable and the best correlation between PCL and FRAP (r > 0.81) and DPPH (r = 0.74) methods was observed. The TPC in honeys was positively correlated with its antioxidant activity measured by PCL (r > 0.76). Used for the first time, PCL assay demonstrated usefulness in determining the antioxidant capacity of honey, divided into water-and lipid-soluble fractions, measured by ACW and ACL kits, respectively.


Honey Antioxidant capacity Photochemiluminescence method PCL 


Compliance with Ethical Standards


This study was funded by PB/KChTŻ.

Conflict of Interest

Monika Wesołowska declares that she has no conflict of interest. Małgorzata Dżugan declares that she has no conflicts of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Chemistry and Food Toxicology, Faculty of Biology and AgricultureUniversity of RzeszówRzeszówPoland

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