Sensitive Monitoring of Amygdalin and 5-Hydroxytryptamine in Food Supplements Using HILIC OH5 Chromatography
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Amygdalin is a cyanogenic glycoside found in pits and kernels of numerous plants especially from the family Rosaceae. Recently, in some countries, food supplements containing amygdalin have been offered for the use in the alternative cancer treatment. The effectivity of amygdalin in the antitumor therapy remained still questionable. Considering the potential toxicity of amygdalin, it is of a high importance to provide the information of its content in available food supplements. A fast hydrophilic interaction liquid chromatography-high performance liquid chromatography (HILIC-HPLC) method was developed for the analysis of amygdalin and 5-hydroxytryptamine (serotonin), using new Ascentis Express OH5 column (100 × 3.0 mm; 2.7 μm). Mobile phase composed of acetonitrile and 10 mM ammonium acetate pH 3.8, 90:10 (v/v) was used. The developed method was applied to the determination of amygdalin and serotonin in capsules containing apricot kernels and sea buckthorn berries as well as to the determination of amygdalin in apricot kernels and serotonin as a minority compound in sea buckthorn samples for food supplements. The method recovery was in the range 95.25–106.09 % for amygdalin and in the range 97.67–104.30 % for 5-hydroxytryptamine, respectively.
KeywordsAmygdalin Serotonin HILIC OH5
The authors would like to acknowledge the financial support of the project GAČR no. 15-10781S. Martina Parmová would like to acknowledge the financial support of specific research, no. SVV 260 184. The authors would like to gratefully acknowledge Lucie Nováková for the idea to use Ascentis Express OH5 column for the simultaneous determination of serotonin and amygdalin.
Compliance with Ethical Standards
This article does not contain any studies with human or animal subjects.
Conflict of Interest
The authors declare that they have no competing interests.
Informed consent was obtained from all individual participants included in the study.
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