Food Analytical Methods

, Volume 9, Issue 6, pp 1849–1856 | Cite as

Sensitive Monitoring of Amygdalin and 5-Hydroxytryptamine in Food Supplements Using HILIC OH5 Chromatography

  • Lucie Havlíková
  • Martina Parmová
  • Petr Chocholouš
  • Petr Solich


Amygdalin is a cyanogenic glycoside found in pits and kernels of numerous plants especially from the family Rosaceae. Recently, in some countries, food supplements containing amygdalin have been offered for the use in the alternative cancer treatment. The effectivity of amygdalin in the antitumor therapy remained still questionable. Considering the potential toxicity of amygdalin, it is of a high importance to provide the information of its content in available food supplements. A fast hydrophilic interaction liquid chromatography-high performance liquid chromatography (HILIC-HPLC) method was developed for the analysis of amygdalin and 5-hydroxytryptamine (serotonin), using new Ascentis Express OH5 column (100 × 3.0 mm; 2.7 μm). Mobile phase composed of acetonitrile and 10 mM ammonium acetate pH 3.8, 90:10 (v/v) was used. The developed method was applied to the determination of amygdalin and serotonin in capsules containing apricot kernels and sea buckthorn berries as well as to the determination of amygdalin in apricot kernels and serotonin as a minority compound in sea buckthorn samples for food supplements. The method recovery was in the range 95.25–106.09 % for amygdalin and in the range 97.67–104.30 % for 5-hydroxytryptamine, respectively.


Amygdalin Serotonin HILIC OH5 



The authors would like to acknowledge the financial support of the project GAČR no. 15-10781S. Martina Parmová would like to acknowledge the financial support of specific research, no. SVV 260 184. The authors would like to gratefully acknowledge Lucie Nováková for the idea to use Ascentis Express OH5 column for the simultaneous determination of serotonin and amygdalin.

Compliance with Ethical Standards

This article does not contain any studies with human or animal subjects.

Conflict of Interest

The authors declare that they have no competing interests.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

12161_2015_362_MOESM1_ESM.doc (54 kb)
ESM 1 (DOC 54 kb)


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Lucie Havlíková
    • 1
  • Martina Parmová
    • 1
  • Petr Chocholouš
    • 1
  • Petr Solich
    • 1
  1. 1.Department of Analytical Chemistry, Faculty of PharmacyCharles University in PragueHradec KrálovéCzech Republic

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