Food Analytical Methods

, Volume 9, Issue 6, pp 1736–1744 | Cite as

Determination of Aminoglycoside Fungicide Validamycin A in Rice Plant by Quick, Easy, Cheap, Effective, Rugged, and Safe Approach Using Ultra High Performance Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry

  • Na Liu
  • Fengshou Dong
  • Jun Xu
  • Xingang Liu
  • Yongquan ZhengEmail author


A rapid, simple, sensitive, and reliable method was developed for the determination of validamycin A residues in rice plants based on a modified quick, easy, cheap, effective, rugged, and safe extraction procedure using ultra high performance liquid chromatography-tandem mass spectrometry. The samples were extracted with methanol/ water (9:1, v/v) and cleaned-up with octadecylsilane, graphitized carbon black and primary secondary amine for the rice hull, rice straw, and brown rice, respectively. The mass spectrometry parameter optimization was performed in multi-reaction monitoring mode, and electrospray ionization in positive mode was selected. Recovery studies were performed in three matrices at three levels (10, 100, and 500 μg/kg) with average recoveries ranging from 77.7 to 94.3 % and relative standard deviations of less than 9.8 % (n = 5) for the analyte. The limits of detections (LODs) for validamycin A were below 0.40 μg/kg, and the limits of quantifications (LOQs) were below 1.32 μg/kg. The effects of different columns on the separation were also investigated. The method was successfully applied in a study of validamycin A residues in rice plants.


Rice plant Validamycin A Pesticide residue Ultra high performance liquid chromatography-electrospray ionization-tandem mass spectrometry 



This work was financially supported by Nation Nature Science Foundation of China (NSFC, 31272071).

Compliance with Ethical Standards

This is an original research article that has neither been published previously nor considered presently for publication elsewhere.

Informed Consent

All authors named in a manuscript are entitled to the authorship and have approved the final version of the submitted manuscript. This article does not contain any studies with human or animal subjects.

Conflict of Interest

Na Liu declares that she has no conflict of interest. Fengshou Dong declares that he has no conflict of interest. Jun Xu declares that she has no conflict of interest. Xingang Liu declares that he has no conflict of interest. Yongquan Zheng declares that he has no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Na Liu
    • 1
    • 2
  • Fengshou Dong
    • 1
  • Jun Xu
    • 1
  • Xingang Liu
    • 1
  • Yongquan Zheng
    • 1
    Email author
  1. 1.Institute of Plant Protection, State Key Laboratory for Biology of Plant Diseases and Insect PestsChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  2. 2.College of Plant ProtectionShenyang Agricultural UniversityShenyangPeople’s Republic of China

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