Food Analytical Methods

, Volume 9, Issue 4, pp 1068–1078 | Cite as

Determination of Acrylamide Content in Refined Wheat Starch (RWS) Based on Dielectric Property (DP) During Deep-Frying Process

  • Yishan Song
  • Jianfeng Lu
  • Yudong Cheng
  • Yinzhe JinEmail author


A detection method based on dielectric property (DP) technique was used to determinate the acrylamide concentration in refined wheat starch (RWS) during frying process, and the quantitative relationship between the acrylamide concentration in the aqueous solution and the dielectric loss at 2.0 GHz was established with a regression coefficient over 0.99 in the detection process. To evaluate the efficiency of the DP method, the detection results were compared with that determinated by high-performance liquid chromatography (HPLC) method, and the results indicated that the DP technique was a more efficient method. The minimum detectable concentrations (MDCs) of acrylamide were 0.3 and 0.9 μg/mL by DP and HPLC, respectively. The ranges of efficiencies of detection method were within the range of 73–103 % for RWS after hot-air drying pretreatment and 72–130 % for RWS after microwave pretreatment in range of the detectable concentration, respectively. Moreover, the correlation equations of acrylamide concentration and water (and oil) content were established, and the experimental values of acrylamide concentration were furthermore compared with the calculated values, and the correlation coefficients of all samples were above 0.93, and the relative root-mean-square error (RRMSE) and relative error (RE) values demonstrated that they are in good agreement. All these results showed that the DP method is suitable and efficient for determining acrylamide content in fried food. In addition, the microwave and hot-air drying methods were selected to pretreat RWS, and the results showed that the microwave treatment was more effective to reduce the acrylamide contents.


Acrylamide concentration Dielectric property Predried treatment Frying process Microwave 



This work was supported by the Science and Technology Commission of Shanghai Municipality (12290502200) and Shanghai University Knowledge Service Platform and Shanghai Ocean University Aquatic Animal Breeding Center (ZFI206).

Conflict of Interest

Yishan Song declares that he has no conflict of interest. Jianfeng Lu declares that he has no conflict of interest. Yudong Cheng declares that he has no conflict of interest. Yinzhe Jin declares that she has no conflict of interest. This article does not contain any studies with human or animal subjects.


  1. Ahmed J, Ramaswamy HS, Raghavan VGS (2007) Dielectric properties of butter in the MW frequency range as affected by salt and temperature. J Food Eng 82:351–358CrossRefGoogle Scholar
  2. Anese M, Nicol MC, Verardo G, Munari M, Mirolo G, Bortolomeazzi R (2014) Effect of vacuum roasting on acrylamide formation and reduction in coffee beans. Food Chem 145:168–172CrossRefGoogle Scholar
  3. Backe WJ, Yingling V, Johnson T (2014) The determination of acrylamide in environmental and drinking waters by large-volume injection-hydrophilic-interaction liquid chromatography and tandem mass spectrometry. J Chromatogr A 1334:72–78CrossRefGoogle Scholar
  4. Banchero M, Pellegrino G, Manna L (2013) Supercritical fluid extraction as a potential mitigation strategy for the reduction of acrylamide level in coffee. J Food Eng 115:292–297CrossRefGoogle Scholar
  5. Bansal N, Dhaliwal AS, Mann KS (2015) Dielectric properties of corn flour from 0.2 to 10 GHz. J Food Eng 166:255–262CrossRefGoogle Scholar
  6. Bartkiene E, Jakobsone I, Juodeikiene G, Vidmantiene D, Pugajeva I, Bartkevics V (2013) Study on the reduction of acrylamide in mixed rye bread by fermentation with bacteriocin-like inhibitory substances producing lactic acid bacteria in combination with Aspergillus niger glucoamylase. Food Control 30:35–40CrossRefGoogle Scholar
  7. Barutcu I, Sahin S, Sumnu G (2009) Acrylamide formation in different batter formulations during microwave frying. LWT - Food Sci Technol 42:17–22CrossRefGoogle Scholar
  8. Bent GA, Maragh P, Dasgupta T (2012) Acrylamide in Caribbean foods—residual levels and their relation to reducing sugar and asparagine content. Food Chem 133:451–457CrossRefGoogle Scholar
  9. Casado FJ, Montaño A, Spitzner D, Carle R (2013) Investigations into acrylamide precursors in sterilized table olives: evidence of a peptic fraction being responsible for acrylamide formation. Food Chem 141:1158–1165CrossRefGoogle Scholar
  10. Cengiz MF, Gündüz CPB (2013) Acrylamide exposure among Turkish toddlers from selected cereal-based baby food samples. Food Chem Toxicol 60:514–519CrossRefGoogle Scholar
  11. Chandrasekaran S, Ramanathan S, Basak T (2013) Microwave food processing—a review. Food Res Int 52:243–261CrossRefGoogle Scholar
  12. Cheng J, Chen X, Zhao S, Zhang Y (2015) Antioxidant-capacity-based models for the prediction of acrylamide reduction by flavonoids. Food Chem 168:90–99CrossRefGoogle Scholar
  13. Delgado RM, Luna-Bárcenas G, Arámbula-Villa G, Azuara E, López-Peréa P, Salazar R (2014) Effect of water activity in tortilla and its relationship on the acrylamide content after frying. J Food Eng 143:1–7CrossRefGoogle Scholar
  14. Demirok E, Kolsarɪcɪ N (2014) Effect of green tea extract and microwave pre-cooking on the formation of acrylamide in fried chicken drumsticks and chicken wings. Food Res Int 63:290–298CrossRefGoogle Scholar
  15. Erdoğdu SB, Palazoğlu TK, Gőkmen V, Senyuva HZ, Ekiz HI (2007) Reduction of acrylamide formation in French fries by microwave pre-cooking of potato strips. J Sci Food Agr 87:133–137CrossRefGoogle Scholar
  16. Franek M, Rubio D, Diblikova I, Rubio F (2014) Analytical evaluation of a high-throughput enzyme-linked immunosorbent assay for acrylamide determination in fried foods. Talanta 123:146–150CrossRefGoogle Scholar
  17. Friedman M, Levin CE (2008) Review of methods for the reduction of dietary content and toxicity of acrylamide. J Agr Food Chem 56:6113–6140CrossRefGoogle Scholar
  18. Guo W, Zhu X, Liu Y, Zhuang H (2010) Sugar and water contents of honey with dielectric property sensing. J Food Eng 97:275–281CrossRefGoogle Scholar
  19. Heredia A, Castelló ML, Argüelles A, Andrés A (2014) Evolution of mechanical and optical properties of French fries obtained by hot air-frying. LWT - Food Sci Technol 57:755–760CrossRefGoogle Scholar
  20. Hu L, Toyoda K, Ihara I (2010) Discrimination of olive oil adulterated with vegetable oils using dielectric spectroscopy. J Food Eng 96:167–171CrossRefGoogle Scholar
  21. Jiao Y, Tang J, Wang S, Koral T (2014) Influence of dielectric properties on the heating rate in free-running oscillator radio frequency systems. J Food Eng 120:197–203CrossRefGoogle Scholar
  22. Kaatze U (1989) Complex permittivity of water as a function of frequency and temperature. J Chem Eng Data 34:371–374CrossRefGoogle Scholar
  23. Kalita D, Holm DG, Jayanty SS (2013) Role of polyphenols in acrylamide formation in the fried products of potato tubers with colored flesh. Food Res Int 54:753–759CrossRefGoogle Scholar
  24. Kang W, Lu J, Cheng Y, Jin Y (2015) Determination of the concentration of alum additive in deep-fried dough sticks using dielectric spectroscopy. J Food Drug Anal In-PressGoogle Scholar
  25. Kim TH, Shin S, Kim KB, Seo WS, Shin JC, Choi JH, Weon KY, Joo SH, Jeong SW, Shin BS (2015) Determination of acrylamide and glycidamide in various biological matrices by liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study. Talanta 131:46–54CrossRefGoogle Scholar
  26. Lim HH, Shin HS (2014) A new derivatization approach with d-cysteine for the sensitive and simple analysis of acrylamide in foods by liquid chromatography-tandem mass spectrometry. J Chromatogra A 1361:117–124CrossRefGoogle Scholar
  27. Liu C, Luo F, Chen D, Qiu B, Tang X, Ke H, Chen X (2014a) Fluorescence determination of acrylamide in heat-processed foods. Talanta 123:95–100CrossRefGoogle Scholar
  28. Liu S, Ogiwara Y, Fukuoka M, Sakai N (2014b) Investigation and modeling of temperature changes in food heated in a flatbed microwave oven. J Food Eng 131:142–153CrossRefGoogle Scholar
  29. López-López A, Beato VM, Sánchez AH, García-García P, Montaño A (2014) Effects of selected amino acids and water-soluble vitamins on acrylamide formation in a ripe olive model system. J Food Eng 120:9–16CrossRefGoogle Scholar
  30. Lu J, Qi L, Guo W, Song Y, Jung YA, Cheng Y, Jin Y (2015) Determination of fluoride concentration in Antarctic krill (Euphausia superba) using dielectric spectroscopy. B Kor Chem Soc In-PressGoogle Scholar
  31. Mariscal M, Bouchon P (2008) Comparison between atmospheric and vacuum frying of apple slices. Food Chem 107:1561–1569CrossRefGoogle Scholar
  32. McKeown MS, Trabelsi S, Tollner EW, Nelson SO (2012) Dielectric spectroscopy measurements for moisture prediction in Vidalia onions. J Food Eng 111:505–510CrossRefGoogle Scholar
  33. Normandin L, Bouchard M, Ayotte P, Blanchet C, Becalski A, Bonvalot Y, Phaneuf D, Lapointe C, Gagné M, Courteau M (2013) Dietary exposure to acrylamide in adolescents from a Canadian urban center. Food Chem Toxicol 57:75–83CrossRefGoogle Scholar
  34. Oginni OC, Sobukola OP, Henshaw FO, Afolabi WAO, Munoz L (2014) Effect of starch gelatinization and vacuum frying conditions on structure development and associated quality attributes of cassava-gluten based snack. Food Structure, In-PressGoogle Scholar
  35. Omar MMA, Wan Ibrahim WAW, Elbashir AA (2014) Sol–gel hybrid methyltrimethoxysilane- tetraethoxysilane as a new dispersive solid-phase extraction material for acrylamide determination in food with direct gas chromatography–mass spectrometry analysis. Food Chem 158:302–309CrossRefGoogle Scholar
  36. Omar MMA, Elbashir AA, Schmitz OJ (2015) Determination of acrylamide in Sudanese food by high performance liquid chromatography coupled with LTQ Orbitrap mass spectrometry. Food Chem 176:342–349CrossRefGoogle Scholar
  37. Oracz J, Nebesny E, Żyżelewicz D (2011) New trends in quantification of acrylamide in food products. Talanta 86:23–34CrossRefGoogle Scholar
  38. Primo-Martín C (2012) Cross-linking of wheat starch improves the crispness of deep-fried battered food. Food Hydrocoll 28:53–58CrossRefGoogle Scholar
  39. Riboldi BP, Vinhas ÁM, Moreira JD (2014) Risks of dietary acrylamide exposure: a systematic review. Food Chem 157:310–322CrossRefGoogle Scholar
  40. Russo MV, Avino P, Centola A, Notardonato I, Cinelli G (2014) Rapid and simple determination of acrylamide in conventional cereal-based foods and potato chips through conversion to 3-[bis(trifluoroethanoyl)amino]-3-oxopropyl trifluoroacetate by gas chromatography coupled with electron capture and ion trap mass spectrometry detectors. Food Chem 146:204–211CrossRefGoogle Scholar
  41. Sosa-Morales ME, Valerio-Junco L, López-Malo A, García HS (2010) Dielectric properties of foods: reported data in the 21st century and their potential applications. LWT-Food Sci Technol 43:1169–1179CrossRefGoogle Scholar
  42. Stadler RH, Blank I, Varga N, Robert F, Hau J, Guy PA, Robert MC, Riediker S (2002) Acrylamide from Maillard reaction products. Nature 419:449–450CrossRefGoogle Scholar
  43. Sun S, Fang Y, Xia Y (2012) A facile detection of acrylamide in starchy food by using a solid extraction-GC strategy. Food Control 26:220–222CrossRefGoogle Scholar
  44. Tuta S, Palazoğlu TK, Gökmen V (2010) Effect of microwave pre-thawing of frozen potato strips on acrylamide level and quality of French fries. J Food Eng 97:261–266CrossRefGoogle Scholar
  45. Urbančič S, Kolar MH, Dimitrijević D, Demšar L, Vidrih R (2014) Stabilisation of sunflower oil and reduction of acrylamide formation of potato with rosemary extract during deep-fat frying. LWT - Food Sci Technol 57:671–678CrossRefGoogle Scholar
  46. Wang Y, Wig TD, Tang J, Hallberg LM (2003) Dielectric properties of foods relevant to RF and microwave pasteurization and sterilization. J Food Eng 57:257–268CrossRefGoogle Scholar
  47. Wang Y, Lau MH, Tang J, Mao R (2004) Kinetics of chemical marker M-1 formation in whey protein gels for developing sterilization processes based on dielectric heating. J Food Eng 64:111–118CrossRefGoogle Scholar
  48. Xu Y, Cui B, Ran R, Liu Y, Chen H, Kai G, Shi J (2014) Risk assessment, formation, and mitigation of dietary acrylamide: current status and future prospects. Food Chem Toxicol 69:1–12CrossRefGoogle Scholar
  49. Xue C, Fukuoka M, Sakai N (2010) Prediction of the degree of starch gelatinization in wheat flour dough during microwave heating. J Food Eng 97:40–45CrossRefGoogle Scholar
  50. Zyzak DV, Sanders RA, Stojanovic M, Tallmadge DH, Eberhardt BL, Ewald DK, Gruber DC, Morsch TR, Strothers MA, Rizzi GP, Villagran MD (2003) Acrylamide formation mechanism in heated foods. J Agr Food Chem 51:4782–4787CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Yishan Song
    • 1
  • Jianfeng Lu
    • 1
  • Yudong Cheng
    • 1
  • Yinzhe Jin
    • 1
    Email author
  1. 1.College of Food Science and TechnologyShanghai Ocean UniversityLingang New CityChina

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