Food Analytical Methods

, Volume 9, Issue 4, pp 942–949 | Cite as

A Highly Sensitive Resonance Rayleigh Scattering Method for the Determination of Vitamin C Based on the Formation of Zirconium Hexacyanoferrate (II) Nanoparticles

  • Huanjun Peng
  • Mingqiong Zhou
  • Jingdong PengEmail author


In pH 2.55 HCl solution, vitamin C (VC) could deoxidize [Fe(CN)6]3− producing [Fe(CN)6]4−, which reacted with Zr (IV) to form Zr[Fe(CN)6] complex, then aggregated to produce {Zr[Fe(CN)6]}n nanoparticles by virtue of hydrophobic force and van der Waals force. The intensity of RRS at 340 nm is directly proportional to the concentration of VC in the range of 0.1–1.45 μg mL−1, and the detection limit (3 s/k) is 0.128 ng mL−1. In this work, the spectral characteristics of RRS, the optimum condition, the reaction mechanism of the reaction, and their influencing factors especially the effect of organic solvent have been studied. The sensitive, rapid, and simple method based on resonance Rayleigh scattering was successfully applied to determine trace amount VC in VC injection and tablet.


Resonance Rayleigh scattering Vitamin C Zr[Fe(CN)6] nanoparticles 



This project was supported by the National Natural Science Foundation of China (No. 20975083), and here, all authors express their deep thanks.

Conflict of Interest

Jingdong Peng declares that he has no conflict of interest. Huanjun Peng declares that she has no conflict of interest. Mingqiong Zhou declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.School of Chemistry and Chemical EngineeringSouthwest UniversityChongqingPeople’s Republic of China

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