Food Analytical Methods

, Volume 9, Issue 3, pp 638–645 | Cite as

Determination of Menbutone in Bovine Milk and Meat Using Micellar Liquid Chromatography: Application to Injectable Dosage Forms

  • F. Belal
  • Sawsan A. Abd El-Razeq
  • Manal M. Fouad
  • S. ZayedEmail author
  • Fatma A. Fouad


A simple, sensitive, and rapid method was developed for the routine identification and quantification of menbutone in different matrices by micellar liquid chromatography. Separation was performed in less than 4 min using a C18 column with UV detection at 234 nm. A micellar solution composed of 0.12 M sodium dodecyl sulfate, 8 % n-butanol, and 0.3 % triethylamine in 0.02 M phosphoric acid at pH 6.0 was used as the mobile phase. The method was fully validated in accordance with International Conference on Harmonization (ICH) guidelines. The limits of detection and quantitation were 0.95 and 2.86 ng mL−1, respectively. The method showed good repeatability, linearity, and sensitivity according to the evaluation of the validation parameters. The micellar method was successfully applied for the analysis of menbutone in its commercial injections with a mean % recovery value of 99.73 ± 1.634 % and in spiked bovine milk and meat samples with a mean % recovery values in the range of 98.00–100.60 %. High extraction efficiency was obtained without matrix interference in the extraction process and in the subsequent chromatographic determination. No organic solvent was used during the pretreatment step. Hence, this method can be considered as an interesting example for green chemistry.


Micellar liquid chromatography Menbutone Dosage forms Bovine milk Meat 


Conflict of Interest

The authors declare no conflict of interest. This article does not contain any studies with human or animal subjects.


  1. Ackerman L (2007) Veterinary practice management. Black Well, New Jersey, p 342Google Scholar
  2. Belal F, El-Brashy A, Eid M, Nasr J (2009) Liq Chromatogr Related Technol 32:2993CrossRefGoogle Scholar
  3. Belal F, Sharaf El-Din M, El-Enany N, Saad S (2013) Chem Cent J 7:162CrossRefGoogle Scholar
  4. Beltran-Martinavarro B, Peris-Vicente J, Marco-Peiro S, Esteve-Romero S, Rambla-Alegre M, Carda-Boch S (2012) Analyst 137:269CrossRefGoogle Scholar
  5. Berthod A, Garcia-Alvarez-Coque MC (2000) 83, Marcel Dekker, New YorkGoogle Scholar
  6. CX/RVDF 06/16/13, Report on 16th session of the Codex Committee on Residues of Veterinary Drugs in Foods (2006) Codex Alimentarius Commission, Jiont FAO/WHO Food Standards Programme, Rome. Accessed 12 May 2006
  7. Fouad M, Abdel-Razeq S, Belal F, Fouad F (2013) Int J Pharm Anal 4:30Google Scholar
  8. Hirosh M, Kouhei F, Toshiaki T (2010) Kumamoto-Ken Hoken Kankyo Kagaku Kenkyushoho 39:21Google Scholar
  9. International Conference on Harmonization (ICH) (2005) Technical requirements for the registration of pharmaceutical for human use: validation of analytical procedures, text and methodology Q2 (R1), Geneva, 1Google Scholar
  10. Kalyankar T, Kulkarini P, Wadher S, Pekamwar S (2014) J Appl Pharm Sci 4:128Google Scholar
  11. Lund J, Lassen J (1969) Acta Pharmacol Toxicol 27:429CrossRefGoogle Scholar
  12. Luo Y, Tan M, Luo L, Wang L, Yang Y, Long F, Wang S, Wu M (2013a) Faming Zhuanli Shenqing CN 103424491 A 12:4Google Scholar
  13. Luo Y, Tan M, Luo L, Wang L, Yang Y, Long F, Wang S, Wu M (2013b) Faming Zhuanli Shenqing CN 103439421 A 12:11Google Scholar
  14. Malinowska I, Stepnik K (2012) Chromatogr Res Int 2012:1CrossRefGoogle Scholar
  15. Miller J, Miller J (2005) Statistics and chemometrics for analytical chemistry, 5th edn. Pearson Education Limited, Harlow, p 256Google Scholar
  16. Mourya SK, Dubey S, Durgabanshi A, Shukia SK, Esteve-Romero J, Bose D (2011) J AOAC Int 94:1082Google Scholar
  17. Nasr J, Shalan S, Belal F (2013a) Chromatographia 76:523CrossRefGoogle Scholar
  18. Nasr J, Shalan S, Belal F (2013b) Food Anal Methods 6:1522CrossRefGoogle Scholar
  19. O’Neil J (2006) The Merck Index, 14th edn. Merck and Co, Inc, Rahway, p 1008Google Scholar
  20. Rambla-Alegre M (2012) Chromatogr Res Int 2012:1Google Scholar
  21. Rizk M, Toubar S, Abou El-Alamin M (2014) Eur J Chem 5:11CrossRefGoogle Scholar
  22. Rodriguez E, Moreno-Bondi MC, Marazuela MD (2008) J Chromatogr A 1209:136CrossRefGoogle Scholar
  23. Shoichiro N, Hisaya T (2008) Nagoya-shi Eisei Kenkyushoho 54:1Google Scholar
  24. Soltani S, Jouyban A (2012) Bioanalysis 4:41CrossRefGoogle Scholar
  25. Symonds HW (1982) Vet Rec 110:423CrossRefGoogle Scholar
  26. Szymanski A (2008) Toxicol Mech Methods 18:473CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • F. Belal
    • 1
  • Sawsan A. Abd El-Razeq
    • 2
  • Manal M. Fouad
    • 2
  • S. Zayed
    • 3
    Email author
  • Fatma A. Fouad
    • 2
  1. 1.Pharmaceutical Analytical Chemistry Department, Faculty of PharmacyUniversity of MansouraMansouraEgypt
  2. 2.Analytical Chemistry Department, Faculty of Pharmacy (Girls)Al-Azhar UniversityCairoEgypt
  3. 3.Unit of Drug Analysis, Faculty of PharmacyUniversity of MansouraMansouraEgypt

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