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Food Analytical Methods

, Volume 9, Issue 3, pp 638–645 | Cite as

Determination of Menbutone in Bovine Milk and Meat Using Micellar Liquid Chromatography: Application to Injectable Dosage Forms

  • F. Belal
  • Sawsan A. Abd El-Razeq
  • Manal M. Fouad
  • S. ZayedEmail author
  • Fatma A. Fouad
Article

Abstract

A simple, sensitive, and rapid method was developed for the routine identification and quantification of menbutone in different matrices by micellar liquid chromatography. Separation was performed in less than 4 min using a C18 column with UV detection at 234 nm. A micellar solution composed of 0.12 M sodium dodecyl sulfate, 8 % n-butanol, and 0.3 % triethylamine in 0.02 M phosphoric acid at pH 6.0 was used as the mobile phase. The method was fully validated in accordance with International Conference on Harmonization (ICH) guidelines. The limits of detection and quantitation were 0.95 and 2.86 ng mL−1, respectively. The method showed good repeatability, linearity, and sensitivity according to the evaluation of the validation parameters. The micellar method was successfully applied for the analysis of menbutone in its commercial injections with a mean % recovery value of 99.73 ± 1.634 % and in spiked bovine milk and meat samples with a mean % recovery values in the range of 98.00–100.60 %. High extraction efficiency was obtained without matrix interference in the extraction process and in the subsequent chromatographic determination. No organic solvent was used during the pretreatment step. Hence, this method can be considered as an interesting example for green chemistry.

Keywords

Micellar liquid chromatography Menbutone Dosage forms Bovine milk Meat 

Notes

Conflict of Interest

The authors declare no conflict of interest. This article does not contain any studies with human or animal subjects.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • F. Belal
    • 1
  • Sawsan A. Abd El-Razeq
    • 2
  • Manal M. Fouad
    • 2
  • S. Zayed
    • 3
    Email author
  • Fatma A. Fouad
    • 2
  1. 1.Pharmaceutical Analytical Chemistry Department, Faculty of PharmacyUniversity of MansouraMansouraEgypt
  2. 2.Analytical Chemistry Department, Faculty of Pharmacy (Girls)Al-Azhar UniversityCairoEgypt
  3. 3.Unit of Drug Analysis, Faculty of PharmacyUniversity of MansouraMansouraEgypt

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