Electrochemical Sensor based on Imprinted Sol-Gel Polymer on Au NPs-MWCNTs-CS Modified Electrode for the Determination of Acrylamide
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A sensitive molecularly imprinted electrochemical sensor was successfully constructed for the detection of acrylamide (AM). It is based on a glassy carbon electrode modified with a composites prepared from gold nanoparticles, multiwalled carbon nanotubes, and chitosan along with a sol-gel-based molecularly imprinted polymer (MIP) film. The latter was prepared using AM as the template molecule, 3-aminopropyltrimethoxysilane as the functional monomer, and tetraethoxysilane as the cross-linker. The MIP sensor showed a linear current response to the target AM concentration in the range from 0.05 to 5 μg mL−1 at a working voltage of 0–0.4 V with a lower detection limit of 0.028 μg mL−1 (S/N = 3). It was successfully applied to the detection of AM in potato chips. HPLC analysis was also conducted to detect AM in the same samples to demonstrate the applicability of the electrochemical MIP sensor.
KeywordsElectrochemical sensor Acrylamide Gold nanoparticles Chitosan Multiwalled carbon nanotubes Sol-gel molecularly imprinted
This work was financially supported by the National Natural Science Foundation of China (No. 31201375), Hunan Provincial Natural Science Foundation of China (No. 2015JJ3077), and Special Fund for Agro-scientific Research in the Public Interest (No. 201303084).
Compliance with Ethics Requirements
This article does not contain any studies with human or animal subjects.
Conflict of Interest
Xia Liu declares that she has no conflict of interest. Lu-Gang Mao declares that he has no conflict of interest. Yuan-Liang Wang declares that he has no conflict of interest. Xing-Bo Shi declares that he has no conflict of interest. Yan Liu declares that she has no conflict of interest. Yang Yang declares that she has no conflict of interest. Zao He declares that she has no conflict of interest.
- Gong X (2014) Facile formation of nanoparticle patterns by water induced flow of a polymer thin film. Res Adv 4:54494Google Scholar
- International Food Safety Authorities Network (INFOSAN) Information Note No. 2. Acrylamide in food is a potential health hazard. Geneva, Switzerland, 1st March, 2005, http://www.fsai.ie/industry/hottopics/EN.note2.2005.acrylamide.Pdf
- Riskin M, Tel-Vered R, Bourenko T, Granot E, Willner I (2008) Imprinting of molecular recognition sites through electropolymerization of functionalized Au nanoparticles: development of an electrochemical TNT Sensor Based on π-Donor − Acceptor Interactions. J Am Chem Soc 130:9726–9733CrossRefGoogle Scholar
- Wang X, Piro B, Reisberg S, Anquetin G, Rocquigny HD, Jiang P, Wang Q, Wu W, Pham MC, Dong CZ (2014a) Direct, reagentless electrochemical detection of the BIR3 domain of X-linked inhibitor of apoptosis protein using a peptide-based conducting polymer sensor. Biosens Bioelectron 61:57–62CrossRefGoogle Scholar
- WTO (1993) Guidelines for drinking water quality [R]. WHO: World Health OrganizationGoogle Scholar
- Yang YK, Fang GZ, Liu GY, Pan MF, Wang XM, Kong LJ, He XL, Wang S (2013) Electrochemical sensor based on molecularly imprinted polymer film via sol-gel technology and multi-walled carbon nanotubes-chitosan functional layer for sensitive determination of quinoxaline-2-carboxylic acid. Biosens Bioelectron 47:475–481CrossRefGoogle Scholar
- Zhu AH, Xu GL, Li L, Yang LL, Zhou H, Kan XW (2013) Sol-gel imprinted polymers based electrochemical sensor for paracetamol recognition and detection. Anal Lett 46:7Google Scholar