Food Analytical Methods

, Volume 8, Issue 9, pp 2185–2193 | Cite as

Curcuminoid’s Content and Fingerprint Analysis for Authentication and Discrimination of Curcuma xanthorrhiza from Curcuma longa by High-Performance Liquid Chromatography-Diode Array Detector

  • Mohamad Rafi
  • Laela Wulansari
  • Rudi Heryanto
  • Latifah Kosim Darusman
  • Lee Wah Lim
  • Toyohide Takeuchi


An accurate and reliable method for authentication and discrimination of Curcuma xanthorrhiza (CX) from Curcuma longa (CL) by determining the curcuminoid’s content and analyzing the HPLC fingerprint combined with discriminant analysis (DA) was developed. By using the proposed method, it was found that CL had higher amount of all curcuminoid compounds compared to CX. Therefore, these two closely related species could be authenticated and discriminated by the amount of curcuminoids present in the samples. Authentication and discrimination of the two species were also achieved by comparing their HPLC fingerprint chromatograms using their typical marker peaks. To be more convincing, an aid from DA was also used. Combination of HPLC fingerprint analysis and DA gave excellent result that the two species were separated clearly, including CX samples adulterated with CL. The developed method was successfully used for quality control of the two plants.


Curcuma xanthorrhiza Curcuma longa Curcuminoids High-performance liquid chromatography Discriminant analysis 


Conflict of Interest

Mohamad Rafi declares that he has no conflict of interest. Laela Wulansari declares that she has no conflict of interest. Rudi Heryanto declares that he has no conflict of interest. Latifah Kosim Darusman declares that she has no conflict of interest. Lee Wah Lim declares that she has no conflict of interest. Toyohide Takeuchi declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Mohamad Rafi
    • 1
    • 2
  • Laela Wulansari
    • 1
  • Rudi Heryanto
    • 1
    • 2
  • Latifah Kosim Darusman
    • 1
    • 2
  • Lee Wah Lim
    • 3
  • Toyohide Takeuchi
    • 3
  1. 1.Department of Chemistry, Faculty of Mathematics and Natural SciencesBogor Agricultural UniversityBogorIndonesia
  2. 2.Biopharmaca Research Center-Research and Community Empowerment InstituteBogor Agricultural UniversityBogorIndonesia
  3. 3.Department of Chemistry and Biomolecular Science, Faculty of EngineeringGifu UniversityGifuJapan

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