Curcuminoid’s Content and Fingerprint Analysis for Authentication and Discrimination of Curcuma xanthorrhiza from Curcuma longa by High-Performance Liquid Chromatography-Diode Array Detector
An accurate and reliable method for authentication and discrimination of Curcuma xanthorrhiza (CX) from Curcuma longa (CL) by determining the curcuminoid’s content and analyzing the HPLC fingerprint combined with discriminant analysis (DA) was developed. By using the proposed method, it was found that CL had higher amount of all curcuminoid compounds compared to CX. Therefore, these two closely related species could be authenticated and discriminated by the amount of curcuminoids present in the samples. Authentication and discrimination of the two species were also achieved by comparing their HPLC fingerprint chromatograms using their typical marker peaks. To be more convincing, an aid from DA was also used. Combination of HPLC fingerprint analysis and DA gave excellent result that the two species were separated clearly, including CX samples adulterated with CL. The developed method was successfully used for quality control of the two plants.
KeywordsCurcuma xanthorrhiza Curcuma longa Curcuminoids High-performance liquid chromatography Discriminant analysis
Conflict of Interest
Mohamad Rafi declares that he has no conflict of interest. Laela Wulansari declares that she has no conflict of interest. Rudi Heryanto declares that he has no conflict of interest. Latifah Kosim Darusman declares that she has no conflict of interest. Lee Wah Lim declares that she has no conflict of interest. Toyohide Takeuchi declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects
- Arora RB, Kapoor V, Basu N, Jain AP (1971) Anti-inflammatory studies on Curcuma longa (turmeric). Indian J Med Res 59:1289–1295Google Scholar
- Chempakam B, Parthasarathy VA (2008) Turmeric. In: Pathasarathy VA, Chempakam B, Zachariah TJ (eds) Chemistry of spices. CAB International, OxfordGoogle Scholar
- International Conference on Harmonization (2005) Validation of analytical procedures: methodology Q2(R1). ICH, Geneva. URL [http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1__Guideline.pdf]. accessed November 2013.
- Ministry of Health Republic of Indonesia (2008) Indonesia herbal pharmacopoeia volume 1. Ministry of Health Republic of Indonesia, JakartaGoogle Scholar
- Pothitirat W, Gritsanapan W (2006) Variation of bioactive components in Curcuma longa in Thailand. Curr Sci 91:1397–1400Google Scholar
- Singh R, Chandra R, Bose M, Luthra PM (2002) Antibacterial activity of Curcuma longa rhizome extract on pathogenic bacteria. Curr Sci 83:737–740Google Scholar